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pECMV-3×Flag-T7 RNA polymerase聚合酶基因质粒

  • 货号：KL-ZL3363-3ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL3363-3ug pECMV-3×Flag-T7 RNA polymerase聚合酶基因质粒 3ug

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》产品信息

货号 名称 规格
KL-ZL3363 pECMV-3×Flag-T7 RNA polymerase聚合酶基因质粒

3ug

》产品描述

原核抗性： 氨苄青霉素Amp 筛选标记： 遗传霉素G418 克隆菌株： 大肠杆菌DH5α 培养条件： 37℃

》产品简介

pECMV-3×FLAG-T7 RNA polymerase是一个哺乳细胞T7 RNA聚合酶表达质粒。

》产品图谱及序列


序列：
CDS complement(7138..7998)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=Amp
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7999..8103)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac
961 atcgactaca aggatgacga tgacaagctt ggtaccgagc tcggatccac tagtccagtg
1021 tggtggaatt ctatgaacac gattaacatc gctaagaacg acttctctga catcgaactg
1081 gctgctatcc cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag
1141 ttggcccttg agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag
1201 cgtcaactta aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc
1261 ctactcccta agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc
1321 ggcaagcgcc cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac
1381 atcaccatta agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct
1441 gtagcaagcg caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac
1501 cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac
1561 gtctacaaga aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc
1621 ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc
1681 atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta
1741 gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc
1801 cgtgcaggtg cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag
1861 ccgtggactg gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg
1921 gtgcgtactc acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg
1981 tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg
2041 gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag
2101 cgtgaagaac tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg
2161 tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc
2221 agccttgagt tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc
2281 ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt
2341 aacgatatga ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt
2401 tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct
2461 gagcgcatca agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca
2521 ctggagaaca cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt
2581 gagtacgctg gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt
2641 gacgggtctt gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt
2701 cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag
2761 aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga agtagttacc
2821 gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg
2881 gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg
2941 ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag
3001 ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac
3061 atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg
3121 aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga
3181 gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg
3241 caggaataca agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc
3301 ttacagccta ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct
3361 ggtatcgctc ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg
3421 tgggcacacg agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacc
3481 attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat
3541 gagtcttgtg atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct
3601 caattggaca aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta
3661 gagtcggact tcgcgttcgc gttcgcgtaa ctcgagtcta gagggcccgt ttaaacccgc
3721 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
3781 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
3841 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
3901 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
3961 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
4021 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
4081 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
4141 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
4201 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
4261 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
4321 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
4381 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
4441 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
4501 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
4561 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
4621 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
4681 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
4741 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
4801 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
4861 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
4921 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
4981 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
5041 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
5101 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
5161 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
5221 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
5281 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
5341 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
5401 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
5461 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
5521 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
5581 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
5641 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
5701 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
5761 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
5821 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
5881 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
5941 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
6001 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
6061 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
6121 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
6181 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
6241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
6301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
6361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
6421 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
6481 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
6541 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
6601 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
6661 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
6721 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
6781 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
6841 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
6901 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
6961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
7021 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
7081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
7141 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
7201 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
7261 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
7321 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
7381 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
7441 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
7501 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
7561 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
7621 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
7681 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
7741 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
7801 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
7861 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
7921 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
7981 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
8041 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
8101 gcgcacattt ccccgaaaag tgccacctga cgtc

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