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p2Luc哺乳报告质粒

  • 货号：KL-ZL3352-3ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL3352-3ug p2Luc哺乳报告质粒 3ug

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》产品信息

货号 名称 规格
KL-ZL3352 p2Luc哺乳报告质粒

3ug

》产品描述

启动子： SV40 复制子： pUC 终止子： SV40 poly(A) signal 原核抗性： 氨苄青霉素Amp 筛选标记： 荧光素酶Luc 克隆菌株： 大肠杆菌DH5α 培养条件： 37℃

》产品简介

p2Luc是一个哺乳细胞双荧光素酶报告质粒。

》产品图谱及序列


序列：
CDS 3711..4571
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4742..5330
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 agatcgatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt
61 accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
181 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
241 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
301 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
421 aaaaagcttg attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga
481 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
541 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
601 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc
661 tagagtactt aatacgactc actataggct agccaccatg acttcgaaag tttatgatcc
721 agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa
781 tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat
841 ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga
901 gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg
961 taatggttct tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct
1021 taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca
1081 ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga
1141 tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc
1201 tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc
1261 aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga
1321 gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg
1381 tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga
1441 tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga
1501 aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca
1561 agaagatgca cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa
1621 aaatgaacaa atgtcgacgg atccttcaac ttccctgagc tcgaagacgc caaaaacata
1681 aagaaaggcc cggcgccatt ctatcctcta gaggatggaa ccgctggaga gcaactgcat
1741 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
1801 gaggtgaaca tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc agaagctatg
1861 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
1921 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
1981 atttataatg aacgtgaatt gctcaacagt atgaacattt cgcagcctac cgtagtgttt
2041 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaaattacc aataatccag
2101 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
2161 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtaccaga gtcctttgat
2221 cgtgacaaaa caattgcact gataatgaat tcctctggat ctactgggtt acctaagggt
2281 gtggcccttc cgcatagaac tgcctgcgtc agattctcgc atgccagaga tcctattttt
2341 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
2401 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
2461 tttgaagaag agctgttttt acgatccctt caggattaca aaattcaaag tgcgttgcta
2521 gtaccaaccc tattttcatt cttcgccaaa agcactctga ttgacaaata cgatttatct
2581 aatttacacg aaattgcttc tgggggcgca cctctttcga aagaagtcgg ggaagcggtt
2641 gcaaaacgct tccatcttcc agggatacga caaggatatg ggctcactga gactacatca
2701 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
2761 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcagaga
2821 ggcgaattat gtgtcagagg acctatgatt atgtccggtt atgtaaacaa tccggaagcg
2881 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
2941 gaagacgaac acttcttcat agttgaccgc ttgaagtctt taattaaata caaaggatat
3001 caggtggccc ccgctgaatt ggaatcgata ttgttacaac accccaacat cttcgacgcg
3061 ggcgtggcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
3121 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
3181 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
3241 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
3301 tccaaattgt aacacgtgta attctagagc ggccgcttcg agcagacatg ataagataca
3361 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
3421 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
3481 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
3541 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc aggtggcact tttcggggaa
3601 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
3661 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
3721 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
3781 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
3841 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
3901 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
3961 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
4021 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
4081 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
4141 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
4201 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
4261 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
4321 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
4381 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
4441 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
4501 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
4561 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
4621 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
4681 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
4741 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
4801 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
4861 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
4921 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
4981 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
5041 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
5101 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
5161 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
5221 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
5281 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
5341 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg gctcgac

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