6-His-MBP-TEV-FnCpf1大肠敲除质粒

序列：

promoter 10081..10185

/gene="bla"

/label=AmpR promoter

CDS 10186..361

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

ORIGIN

1 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac

61 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga

121 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg

181 gtttattgct gataaatctg gagccggtga gcgtggtagt cgcggtatca ttgcagcact

241 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac

301 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta

361 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt

421 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga

481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc

541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt

601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc

661 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc

721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg

781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg

841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga

901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc

961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg

1021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg

1081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt

1141 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc

1201 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg

1261 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt

1321 tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag tacaatctgc

1381 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg

1441 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg

1501 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac

1561 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg

1621 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta

1681 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg

1741 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga

1801 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg

1861 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc

1921 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc

1981 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac

2041 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc

2101 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc

2161 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggggcc gccatgccgg

2221 cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag

2281 cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc

2341 gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca

2401 tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg

2461 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga

2521 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt

2581 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc

2641 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg

2701 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt

2761 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact

2821 accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc

2881 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc

2941 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga

3001 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa

3061 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg

3121 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag

3181 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg

3241 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc

3301 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc

3361 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga

3421 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg

3481 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa

3541 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct

3601 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg

3661 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg

3721 ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag

3781 caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac

3841 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg

3901 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt

3961 gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaattaata

4021 cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt

4081 aactttaaga aggagatata catatgggtc atcatcatca ccatcacaaa atcgaagaag

4141 gcaaactggt tatttggatc aatggcgata aaggctataa tggtctggca gaagttggca

4201 aaaaattcga aaaagatacc ggcattaaag tgaccgttga acatccggat aaactggaag

4261 aaaaatttcc gcaggttgca gcaaccggtg atggtccgga tattatcttt tgggcacatg

4321 atcgttttgg tggttatgca cagagcggtc tgctggcaga aattacaccg gataaagcat

4381 ttcaggacaa actgtatccg tttacctggg atgcagttcg ctataacggt aaactgattg

4441 catatccgat tgcagttgaa gcactgagcc tgatctataa caaagatctg ctgccgaatc

4501 cgcctaaaac ctgggaagaa attccggcac tggataaaga actgaaagca aaaggtaaaa

4561 gcgcactgat gtttaatctg caagaaccgt attttacctg gcctctgatt gcagcagatg

4621 gtggctatgc attcaaatat gaaaacggca aatatgatat caaagacgtg ggtgttgata

4681 atgcgggtgc aaaagccggt ctgacctttc tggttgatct gattaaaaac aaacacatga

4741 acgccgatac cgattatagc attgcagaag cagcatttaa caaaggtgaa accgcaatga

4801 caattaatgg tccgtgggca tggtcaaata ttgataccag caaagtgaat tatggtgtta

4861 ccgttctgcc gacatttaaa ggtcagccga gcaaaccgtt tgttggtgtt ctgagcgcag

4921 gtattaatgc agcaagcccg aacaaagaac tggcaaaaga atttctggaa aactatctgc

4981 tgaccgatga aggtctggaa gcagtgaata aagataaacc gctgggtgca gttgcactga

5041 aaagctatga agaagaactg gttaaagatc cgcgtattgc agccaccatg gaaaatgcac

5101 agaaaggcga aattatgccg aatattccgc agatgagcgc attttggtat gccgttcgta

5161 ccgcagtgat taatgccgca tcaggtcgtc agaccgttga tgaagccctg aaagatgccc

5221 agaccggtgc aagcaccgga tccctggttc cgcgtggtag cgctagcgaa aatctgtatt

5281 ttcagggaat gagcatctac caggagttcg tcaacaagta ttcactgagt aagacactgc

5341 ggttcgagct gatcccacag ggcaagacac tggagaacat caaggcccga ggcctgattc

5401 tggacgatga gaagcgggca aaagactata agaaagccaa gcagatcatt gataaatacc

5461 accagttctt tatcgaggaa attctgagct ccgtgtgcat cagtgaggat ctgctgcaga

5521 attactcaga cgtgtacttc aagctgaaga agagcgacga tgacaacctg cagaaggact

5581 tcaagtccgc caaggacacc atcaagaaac agattagcga gtacatcaag gactccgaaa

5641 agtttaaaaa tctgttcaac cagaatctga tcgatgctaa gaaaggccag gagtccgacc

5701 tgatcctgtg gctgaaacag tctaaggaca atgggattga actgttcaag gctaactccg

5761 atatcactga tattgacgag gcactggaaa tcatcaagag cttcaaggga tggaccacat

5821 actttaaagg cttccacgag aaccgcaaga acgtgtactc cagcaacgac attcctacct

5881 ccatcatcta ccgaatcgtc gatgacaatc tgccaaagtt cctggagaac aaggccaaat

5941 atgaatctct gaaggacaaa gctcccgagg caattaatta cgaacagatc aagaaagatc

6001 tggctgagga actgacattc gatatcgact ataagactag cgaggtgaac cagagggtct

6061 tttccctgga cgaggtgttt gaaatcgcca atttcaacaa ttacctgaac cagtccggca

6121 ttactaaatt caataccatc attggcggga agtttgtgaa cggggagaat accaagcgca

6181 agggaattaa cgaatacatc aatctgtata gccagcagat caacgacaaa actctgaaga

6241 aatacaagat gtctgtgctg ttcaaacaga tcctgagtga taccgagtcc aagtcttttg

6301 tcattgataa actggaagat gactcagacg tggtcactac catgcagagc ttttatgagc

6361 agatcgccgc tttcaagaca gtggaggaaa aatctattaa ggaaactctg agtctgctgt

6421 tcgatgacct gaaagcccag aagctggacc tgagtaagat ctacttcaaa aacgataaga

6481 gtctgacaga cctgtcacag caggtgtttg atgactattc cgtgattggg accgccgtcc

6541 tggagtacat tacacagcag atcgctccaa agaacctgga taatccctct aagaaagagc

6601 aggaactgat cgctaagaaa accgagaagg caaaatatct gagtctggaa acaattaagc

6661 tggcactgga ggagttcaac aagcacaggg atattgacaa acagtgccgc tttgaggaaa

6721 tcctggccaa cttcgcagcc atccccatga tttttgatga gatcgcccag aacaaagaca

6781 atctggctca gatcagtatt aagtaccaga accagggcaa gaaagacctg ctgcaggctt

6841 cagcagaaga tgacgtgaaa gccatcaagg atctgctgga ccagaccaac aatctgctgc

6901 acaagctgaa aatcttccat attagtcagt cagaggataa ggctaatatc ctggataaag

6961 acgaacactt ctacctggtg ttcgaggaat gttacttcga gctggcaaac attgtccccc

7021 tgtataacaa gattaggaac tacatcacac agaagcctta ctctgacgag aagtttaaac

7081 tgaacttcga aaatagtacc ctggccaacg ggtgggataa gaacaaggag cctgacaaca

7141 cagctatcct gttcatcaag gatgacaagt actatctggg agtgatgaat aagaaaaaca

7201 ataagatctt cgatgacaaa gccattaagg agaacaaagg ggaaggatac aagaaaatcg

7261 tgtataagct gctgcccggc gcaaataaga tgctgcctaa ggtgttcttc agcgccaaga

7321 gtatcaaatt ctacaaccca tccgaggaca tcctgcggat tagaaatcac tcaacacata

7381 ctaagaacgg gagcccccag aagggatatg agaaatttga gttcaacatc gaggattgca

7441 ggaagtttat tgacttctac aagcagagca tctccaaaca ccctgaatgg aaggattttg

7501 gcttccggtt ttccgacaca cagagatata actctatcga cgagttctac cgcgaggtgg

7561 aaaatcaggg gtataagctg acttttgaga acatttctga aagttacatc gacagcgtgg

7621 tcaatcaggg aaagctgtac ctgttccaga tctataacaa agatttttca gcatacagca

7681 agggcagacc aaacctgcat acactgtact ggaaggccct gttcgatgag aggaatctgc

7741 aggacgtggt ctataaactg aacggagagg ccgaactgtt ttaccggaag cagtctattc

7801 ctaagaaaat cactcaccca gctaaggagg ccatcgctaa caagaacaag gacaatccta

7861 agaaagagag cgtgttcgaa tacgatctga ttaaggacaa gcggttcacc gaagataagt

7921 tctttttcca ttgtccaatc accattaact tcaagtcaag cggcgctaac aagttcaacg

7981 acgagatcaa tctgctgctg aaggaaaaag caaacgatgt gcacatcctg agcattgacc

8041 gaggagagcg gcatctggcc tactataccc tggtggatgg caaagggaat atcattaagc

8101 aggatacatt caacatcatt ggcaatgacc ggatgaaaac caactaccac gataaactgg

8161 ctgcaatcga gaaggataga gactcagcta ggaaggactg gaagaaaatc aacaacatta

8221 aggagatgaa ggaaggctat ctgagccagg tggtccatga gattgcaaag ctggtcatcg

8281 aatacaatgc cattgtggtg ttcgaggatc tgaacttcgg ctttaagagg gggcgcttta

8341 aggtggaaaa acaggtctat cagaagctgg agaaaatgct gatcgaaaag ctgaattacc

8401 tggtgtttaa agataacgag ttcgacaaga ccggaggcgt cctgagagcc taccagctga

8461 cagctccctt tgaaactttc aagaaaatgg gaaaacagac aggcatcatc tactatgtgc

8521 cagccggatt cacttccaag atctgccccg tgaccggctt tgtcaaccag ctgtacccta

8581 aatatgagtc agtgagcaag tcccaggaat ttttcagcaa gttcgataag atctgttata

8641 atctggacaa ggggtacttc gagttttcct tcgattacaa gaacttcggc gacaaggccg

8701 ctaaggggaa atggaccatt gcctccttcg gatctcgcct gatcaacttt cgaaattccg

8761 ataaaaacca caattgggac actagggagg tgtacccaac caaggagctg gaaaagctgc

8821 tgaaagacta ctctatcgag tatggacatg gcgaatgcat caaggcagcc atctgtggcg

8881 agagtgataa gaaatttttc gccaagctga cctcagtgct gaatacaatc ctgcagatgc

8941 ggaactcaaa gaccgggaca gaactggact atctgattag ccccgtggct gatgtcaacg

9001 gaaacttctt cgacagcaga caggcaccca aaaatatgcc tcaggatgca gacgccaacg

9061 gggcctacca catcgggctg aagggactga tgctgctggg ccggatcaag aacaatcagg

9121 aggggaagaa gctgaacctg gtcattaaga acgaggaata cttcgagttt gtccagaata

9181 gaaataacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagggat

9241 cctacccata cgatgttcca gattacgctt atccctacga cgtgcctgat tatgcatacc

9301 catatgatgt ccccgactat gcctaaagcc tcgagggtgg tggtagcgat tataaagatg

9361 atgatgataa aggcctgaac gatatctttg aagcccagaa aattgaatgg cacgagtaaa

9421 agcttgtcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg

9481 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta

9541 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga

9601 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc

9661 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac

9721 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag

9781 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc

9841 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg

9901 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata

9961 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa

10021 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg

10081 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga

10141 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat

10201 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca

10261 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc

10321 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca

10381 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg

10441 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca

10501 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata

10561 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag

10621 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg

10681 gagct