终止子： OCS terminator
质粒标签： C-HA
原核抗性： 卡那霉素Kan
筛选标记： 草铵膦Bar
克隆菌株： 大肠杆菌DB3.1
培养条件： 37℃
pEarleyGate 301(C-HA)是一个植物细胞Gateway系统表达质粒。pEarleyGate 301 allows for rapid recombinational cloning of DNA from Gateway entry vectors to be epitope tagged with HA tag at the C-terminus of the encoded protein. This vector allows for expression of genes from a promoter of choice rather than from the enhanced 35S promoter (see pEarleyGate 101-105 or 201-204).
序列：
CDS complement(9161..9955)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=Kan
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
1 gctggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
61 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt
121 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag
181 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg
241 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg
301 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt
361 ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg
421 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
481 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
541 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
601 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc
661 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg
721 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
781 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
841 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
901 tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa
961 attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat
1021 ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt
1081 gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta
1141 ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc
1201 gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa
1261 aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga
1321 attgaattct gcagtcgacg gtaccgcggg cccgggatca tcaacaagtt tgtacaaaaa
1381 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
1441 aaaacagact acataatact gtaaaacaca acatatccag tcatattggc ggccgcatta
1501 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
1561 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
1621 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
1681 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
1741 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
1801 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
1861 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
1921 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
1981 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
2041 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
2101 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
2161 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
2221 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
2281 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
2341 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
2401 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
2461 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
2521 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
2581 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
2641 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
2701 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
2761 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
2821 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
2881 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
2941 gaccatagtg actggatatg ttgtgtttta cagcattatg tagtctgttt tttatgcaaa
3001 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
3061 aaagtggtgt acccatacga tgttccagat tacgcttgac taggtgagtc tagagagtta
3121 attaagaccc gggactagtc cctagagtcc tgctttaatg agatatgcga gacgcctatg
3181 atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta
3241 gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg
3301 tatttttatg aataatattc tccgttcaat ttactgattg taccctacta cttatatgta
3361 caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca tctatgatag
3421 agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta aaaaaagcgg
3481 cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt caaaagtgcc
3541 ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac tgaagggaac
3601 tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt ggccgtccgc
3661 tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg gtaaccgact
3721 tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa tgaagtgcag
3781 gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac
3841 atgtcgaggc tcagcaggac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa
3901 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
3961 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
4021 agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt
4081 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
4141 gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
4201 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
4261 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
4321 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
4381 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
4441 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
4501 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
4561 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
4621 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
4681 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
4741 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
4801 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
4861 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
4921 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
4981 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
5041 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
5101 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
5161 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
5221 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
5281 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
5341 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
5401 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
5461 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
5521 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
5581 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
5641 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
5701 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
5761 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
5821 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
5881 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
5941 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
6001 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
6061 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
6121 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
6181 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
6241 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
6301 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
6361 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
6421 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
6481 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
6541 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
6601 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
6661 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
6721 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
6781 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
6841 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
6901 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
6961 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
7021 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
7081 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
7141 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
7201 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
7261 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
7321 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
7381 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
7441 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
7501 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
7561 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
7621 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
7681 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
7741 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
7801 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
7861 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
7921 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
7981 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
8041 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
8101 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
8161 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
8221 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
8281 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
8341 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
8401 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
8461 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
8521 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
8581 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
8641 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
8701 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
8761 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
8821 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
8881 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
8941 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
9001 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
9061 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
9121 aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta
9181 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
9241 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
9301 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
9361 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
9421 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
9481 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
9541 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
9601 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
9661 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
9721 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
9781 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
9841 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
9901 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt
9961 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
10021 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
10081 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg
10141 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
10201 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
10261 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
10321 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctgg
