pCDNA3-HA-p53人源基因哺乳表达质粒

  • 货号:KL-ZL3159-3ug
  • 培养条件:37℃,有氧,LB 序列
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KL-ZL3159-3ug pCDNA3-HA-p53人源基因哺乳表达质粒 3ug

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货号 名称 规格
KL-ZL3159 pCDNA3-HA-p53人源基因哺乳表达质粒

3ug

pCDNA3-HA-p53人源基因哺乳表达质粒
pCDNA3-HA-p53人源基因哺乳表达质粒
质粒大小: 6630bp
质粒标签: N-HA
原核抗性: 氨苄青霉素Amp
筛选标记: 新霉素Neo/G418
克隆菌株: DH5α等大肠杆菌
培养条件: 37℃,有氧,LB

序列:
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttaccatg
901 gcctacccct acgacgtgcc cgactacgcc tccctcggat ccgaggagcc gcagtcagat
961 cctagcgtcg agccccctct gagtcaggaa acattttcag acctatggaa actacttcct
1021 gaaaacaacg ttctgtcccc cttgccgtcc caagcaatgg atgatttgat gctgtccccg
1081 gacgatattg aacaatggtt cactgaagac ccaggtccag atgaagctcc cagaatgcca
1141 gaggctgctc cccccgtggc ccctgcacca gcagctccta caccggcggc ccctgcacca
1201 gccccctcct ggcccctgtc atcttctgtc ccttcccaga aaacctacca gggcagctac
1261 ggtttccgtc tgggcttctt gcattctggg acagccaagt ctgtgacttg cacgtactcc
1321 cctgccctca acaagatgtt ttgccaactg gccaagacct gccctgtgca gctgtgggtt
1381 gattccacac ccccgcccgg cacccgcgtc cgcgccatgg ccatctacaa gcagtcacag
1441 cacatgacgg aggttgtgag gcgctgcccc caccatgagc gctgctcaga tagcgatggt
1501 ctggcccctc ctcagcatct tatccgagtg gaaggaaatt tgcgtgtgga gtatttggat
1561 gacagaaaca cttttcgaca tagtgtggtg gtgccctatg agccgcctga ggttggctct
1621 gactgtacca ccatccacta caactacatg tgtaacagtt cctgcatggg cggcatgaac
1681 cggaggccca tcctcaccat catcacactg gaagactcca gtggtaatct actgggacgg
1741 aacagctttg aggtgcgtgt ttgtgcctgt cctgggagag accggcgcac agaggaagag
1801 aatctccgca agaaagggga gcctcaccac gagctgcccc cagggagcac taagcgagca
1861 ctgcccaaca acaccagctc ctctccccag ccaaagaaga aaccactgga tggagaatat
1921 ttcacccttc agatccgtgg gcgtgagcgc ttcgagatgt tccgagagct gaatgaggcc
1981 ttggaactca aggatgccca ggctgggaag gagccagggg ggagcagggc tcactccagc
2041 cacctgaagt ccaaaaaggg tcagtctacc tcccgccata aaaaactcat gttcaagaca
2101 gaagggcctg actcagactg agaattctgc agatatccat cacactggcg gccgctcgag
2161 catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc
2221 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
2281 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
2341 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
2401 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
2461 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
2521 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
2581 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
2641 tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
2701 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
2761 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
2821 actcaaccct atctcggtct attcttttga tttataaggg attttgggga tttcggccta
2881 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
2941 tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta tgcaaagcat
3001 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
3061 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
3121 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
3181 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
3241 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
3301 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
3361 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
3421 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
3481 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
3541 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
3601 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
3661 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
3721 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
3781 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
3841 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
3901 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
3961 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
4021 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
4081 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
4141 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
4201 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
4261 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
4321 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
4381 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
4441 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
4501 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
4561 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
4621 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
4681 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
4741 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
4801 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
4861 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
4921 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
4981 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
5041 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
5101 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
5161 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
5221 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
5281 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
5341 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
5401 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
5461 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
5521 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
5581 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
5641 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
5701 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
5761 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
5821 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
5881 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
5941 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
6001 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
6061 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
6121 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
6181 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
6241 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
6301 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
6361 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
6421 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
6481 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
6541 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
6601 acatttcccc gaaaagtgcc acctgacgtc

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