培养条件：
LB培养基，37℃
序列：
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa ccggtgcggc cgcataggat
3541 ccagatctag attcaccggt cgcccctctc cctccccccc ccctaacgtt actggccgaa
3601 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
3661 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
3721 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
3781 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
3841 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
3901 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
3961 tcacctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
4021 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg
4081 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataataccg
4141 gtcgatccac aacctgtaca gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
4201 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
4261 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
4321 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
4381 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
4441 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
4501 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
4561 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
4621 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
4681 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
4741 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
4801 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
4861 tcggcatgga cgagctgtac aaggaattct aatctagaac gcgattaatt ctaccgggta
4921 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
4981 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
5041 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
5101 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
5161 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
5221 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
5281 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
5341 ggcccggcat tctgcacgct tcgaagcgca cgtctgccgc gctgttctcc tcttcctcat
5401 ctccgggcct ttcgaattca agggcagagg aagtcttcta acttgaggtg acgtggagga
5461 gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
5521 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
5581 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
5641 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
5701 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
5761 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
5821 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
5881 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
5941 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
6001 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
6061 gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat
6121 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
6181 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
6241 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
6301 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
6361 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
6421 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
6481 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
6541 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
6601 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
6661 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat
6721 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
6781 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
6841 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc
6901 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
6961 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
7021 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
7081 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
7141 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
7201 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
7261 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
7321 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
7381 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
7441 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
7501 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
7561 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct
7621 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
7681 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
7741 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
7801 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
7861 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
7921 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
7981 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
8041 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
8101 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
8161 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
8221 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
8281 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
8341 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
8401 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
8461 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
8521 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
8581 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
8641 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
8701 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
8761 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
8821 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
8881 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
8941 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
9001 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
9061 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
9121 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
9181 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
9241 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
9301 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
9361 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
9421 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
9481 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
9541 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
9601 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
9661 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
9721 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
9781 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
9841 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
9901 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
9961 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
10021 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
10081 aaattgtatt tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt
10141 taaatatgta ctacaaactt agtagt
