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pMXs-miR-GFP-Puro逆转录表达质粒

货号：KL-ZL3129-3ug
培养条件：37℃，有氧 LB

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL3129-3ug	pMXs-miR-GFP-Puro逆转录表达质粒	3ug		立即咨询	+ -

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》产品信息

货号	名称	规格
KL-ZL3129	pMXs-miR-GFP-Puro逆转录表达质粒	3ug

pMXs-miR-GFP/Puro EF-1 Promoter ensures - a high level of expression in mammalian cellsFusion Marker to - monitor cells positive for expression GFP-Puro and stable selection withGFP or puromycin resistance. either

》产品图谱及序列

启动子： EF-1α core promoter

复制子： pUC ori

质粒分类：病毒系列，逆病毒克隆克隆载体

质粒大小： 7001bp

原核抗性： Amp

筛选标记： Puro

克隆菌株： Stbl3

培养条件： 37℃，有氧 LB

表达宿主：哺乳细胞

诱导方式：无须诱导，瞬时表达

5'测序引物：根据序列设计引物

3'测序引物：根据序列设计引物
序列：

ORIGIN

1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta

61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc

121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag

181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat

241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg

301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct

361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg

421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc

481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc

541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag

601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg

661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg

721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg

781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc

841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg

901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct

961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc

1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt

1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa

1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac

1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa

1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc

1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt

1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc

1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc

1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa

1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca

1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca

1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg

1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac

1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acctggcacg

1861 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctcc

1921 ccaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac

1981 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt

2041 ttatcgatgc ctccccgtca ccaccccccc caacccgccc cgaccggagc tgagagtaat

2101 tcatacaaaa ggactcgccc ctgccttggg gaatcccagg gaccgtcgtt aaactcccac

2161 taacgtagaa cccagagatc gctgcgttcc cgccccctca cccgcccgct ctcgtcatca

2221 ctgaggtgga gaagagcatg cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc

2281 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag

2341 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg

2401 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt

2461 tgccgccaga acacaggtac acatattgac caaatcaggg taattttgca tttgtaattt

2521 taaaaaatgc tttcttcttt taatatactt ttttgtttat cttatttcta atactttccc

2581 taatctcttt ctttcagggc aataatgata caatgtatca tgcctctttg caccattcta

2641 aagaataaca gtgataattt ctgggttaag gcaatagccg attagttctc gaggatccga

2701 ctgaagtcgc tagctcgagc ttttggagaa tatttctgca tataaatatt tctgcatata

2761 aattgtaact gatgtaagag gtttcatatt gctaatagca gctacaatcc agctaccatt

2821 ctgcttttat tttatggttg ggataaggct ggattattct gagtccaagc taggcccttt

2881 tgctaatcat gttcatacct cttatcttcc tcccacagct cctgggcaac gtgctggtct

2941 gtgtgctggc ccatcacttt ggcaaagcac gtgagatctg aattctgaca caccatggtg

3001 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac

3061 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag

3121 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg

3181 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac

3241 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag

3301 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac

3361 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg

3421 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc

3481 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac

3541 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg

3601 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg

3661 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggccaccatg

3721 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc

3781 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc

3841 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc

3901 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc

3961 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc

4021 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc

4081 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc

4141 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag

4201 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac

4261 gtcgagtgcc cgaaggaccg cgcgacctgg tgcatgaccc gcaagcccgg tgcctgagcg

4321 gccgccagca cagtggtcga cgataaaata aaagatttta tttagtctcc agaaaaaggg

4381 gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa

4441 ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg

4501 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg

4561 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc

4621 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt

4681 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt

4741 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc

4801 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc

4861 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg

4921 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcacatgc

4981 agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt

5041 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg

5101 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc

5161 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt

5221 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc

5281 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa

5341 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc

5401 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg

5461 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc

5521 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc

5581 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca

5641 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact

5701 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg

5761 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt

5821 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct

5881 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga

5941 gattatcaaa aaggatcttc acctagatcc ttttgcggcc ggccgcaaat caatctaaag

6001 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc

6061 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac

6121 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc

6181 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg

6241 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag

6301 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc

6361 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac

6421 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag

6481 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac

6541 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg

6601 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc

6661 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact

6721 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg

6781 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa

6841 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt

6901 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg

6961 tatttagaaa aataaacaaa taggggttcc gcgcacattt c

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pMXs-miR-GFP-Puro逆转录表达质粒

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