培养条件：
37℃
二,质粒简介：
pENTR223-GLIPR1L1是一个人源基因克隆质粒，Homo sapiens GLIPR1 like 1 (GLIPR1L1), transcript variant 2, mRNA
三,质粒图谱及序列：
序列：
rep_origin 2839..3427
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
661 agttggcatg gctctgaaga ataaattcag ttgtttatgg atcttgggtc tgtgtttggt
721 agccactaca tcttccaaaa tcccatccat cactgaccca cactttatag acaactgcat
781 agaagcccac aacgaatggc gtggcaaagt caaccctccc gcggccgaca tgaaatacat
841 gatttgggat aaaggtttag caaagatggc taaagcatgg gcaaaccagt gcaaatttga
901 acataatgac tgtttggata aatcatataa atgctatgca gcttttgaat atgttggaga
961 aaatatctgg ttaggtggaa taaagtcatt cacaccaaga catgccatta cggcttggta
1021 taatgaaacc caattttatg attttgatag tctatcatgc tccagagtct gtggccatta
1081 tacacagtta gtttgggcca attcatttta tgtcggttgt gcagttgcaa tgtgtcctaa
1141 ccttggggga gcttcaactg caatatttgt atgcaactac ggacctgcag gaaattttgc
1201 aaatatgcct ccttacgtaa gaggagaatc ttgctctctc tgctcaaaag aagagaaatg
1261 tgtaaagaac ctctgcaaaa atccatttct gaagccaacg gggagagcac ctcagcagac
1321 agcctttaat ccattcagct taggttttct tcttctgaga attttttacc taactttctt
1381 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
1441 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
1501 ttacttggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
1561 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
1621 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
1681 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
1741 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
1801 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
1861 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
1921 gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt
1981 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
2041 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
2101 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
2161 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
2221 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
2281 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
2341 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
2401 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
2461 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
2521 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
2581 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
2641 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
2701 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
2761 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
2821 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
2881 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
2941 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
3001 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
3061 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
3121 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
3181 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
3241 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
3301 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
3361 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
3421 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
3481 tcacatgtt
