培养条件：
37℃
二,质粒简介：
pUG34是一个酵母表达质粒。
三,质粒图谱及序列：
序列：
promoter complement(5584..5688)
/gene="bla"
/label=AmpR promoter
misc_feature 5725..6228
/label=CEN/ARS
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgcgtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag
841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat
1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt
1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt
1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cggccgcaaa ttaaagcctt
2101 cgagcgtccc aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg
2161 tctgtacaga aaaaaaagaa aaatttgaaa tataaataac gttcttaata ctaacataac
2221 tataaaaaaa taaataggga cctagacttc aggttgtcta actccttcct tttcggttag
2281 agcggatgtg gggggagggc gtgaatgtaa gcgtgacata actaattaca tgactcgagg
2341 tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta
2401 gatttgtaca attcatccat accatgggta ataccagcag cagtaacaaa ttctaacaag
2461 accatgtggt ctctcttttc gtttggatct ttggataagg cagattgagt ggataagtaa
2521 tggttgtctg gtaacaagac tggaccatca ccaattggag tattttgttg ataatggtca
2581 gctaattgaa cagaaccatc ttcaatgttg tgtctaattt tgaagttaac tttgatacca
2641 ttcttttgtt tgtcagccat gatgtaaaca ttgtgagagt tatagttgta ttccaatttg
2701 tgacctaaaa tgttaccatc ttctttaaaa tcaatacctt ttaattcgat tctattaact
2761 aaggtatcac cttcaaactt gacttcagct ctggtcttgt agttaccgtc atctttgaaa
2821 aaaatagttc tttcttgaac ataaccttct ggcatggcag acttgaaaaa gtcatgttgt
2881 ttcatatgat ctgggtatct cgcaaaacat tgaacaccat aaccgaaagt agtgactaag
2941 gttggccatg gaactggcaa tttaccagta gtacaaataa attttaaggt caatttaccg
3001 taagtagcat caccttcacc ttcaccggag acagaaaatt tgtgaccatt aacatcacca
3061 tctaattcaa ccaaaattgg gacaacacca gtgaataatt cttcaccttt agacattcta
3121 gagtatggat gggggtaata gaattgtatc tatgtatctg acgaccctgt attacacgaa
3181 ggaaatagta gatgaaaaga caagagagca agaaaaagga aaaacttcct ttctaacaga
3241 cgctttactt aaccttatat atatcttatt tttttcatcg agcgtgttca attggacaag
3301 gtgccacctt ttcgacactt cggttattat gttacacagt tttcatgagg atggcgcctt
3361 gactaactta attagtcatt tgccaaccac cacagttccc caatatcgac agcttcacgt
3421 gccatttgcc attttgcgat catgtgacct caagagaaaa agcaaaaaat aatgagagct
3481 aagggattcg aacccttgca tccggagctc cagcttttgt tccctttagt gagggttaat
3541 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
3601 aattccacac aacataggag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
3661 gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
3721 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
3781 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
3841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
3901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
3961 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
4021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
4081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
4141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
4201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
4261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
4321 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
4381 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
4441 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
4501 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
4561 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
4621 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
4681 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
4741 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
4801 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
4861 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
4921 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
4981 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
5041 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
5101 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
5161 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
5221 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
5281 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
5341 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
5401 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
5461 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
5521 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
5581 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
5641 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
5701 aaaagtgcca cctgggtcct tttcatcacg tgctataaaa ataattataa tttaaatttt
5761 ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt
5821 ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac taccttttat
5881 cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct gtgtagaaga
5941 ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat gtatatctat
6001 ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg ttgaaatttt
6061 ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc tttatttact
6121 ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt cccaaattat
6181 tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatccg tcctaagaaa
6241 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
