Available separately or as part of the BacPAK™ Baculovirus Expression System (Cat. No. 631402). pBacPAK9 is a transfer vector designed for high-level expression of a cloned gene driven by the strong AcMNPV polyhedrin promoter. Flanking AcMNPV sequences allow recombination with viral DNA to transfer the expression cassette to the polyhedrin locus of the viral DNA. The polyhedrin coding sequences have been replaced by a multiple cloning site with 18 unique sites which facilitate the insertion of foreign genes in the correct orientation for expression. The Pac I site at the end of the MCS region provides translational stop codons in all three reading frames for expression of truncated proteins. pBacPAK9 has a pUC origin of replication, an M13 origin for single-stranded DNA production, and an ampicillin resistance gene for selection in E. coli.
序列：
ORIGIN
1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
1141 aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
1201 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatcccgg
1261 gaattcgagc tcggtaccag atcttctaga ttcgaactcg aggcctgcag ggcggccgct
1321 taattaattg atccgggtta ttagtacatt tattaagcgc tagattctgt gcgttgttga
1381 tttacagaca attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg
1441 tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa
1501 tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa
1561 ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt
1621 agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg
1681 tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga
1741 ctcgacgtaa acacgttaaa taaagcttgg acatatttaa catcgggcgt gttagcttta
1801 ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat
1861 tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt
1921 gtagttgagc tttttggaat tatttctgat tgcgggcgtt tttgggcggg tttcaatcta
1981 actgtgcccg attttaattc agacaacacg ttagaaagcg atggtgcagg cggtggtaac
2041 atttcagacg gcaaatctac taatggcggc ggtggtggag ctgatgataa atctaccatc
2101 ggtggaggcg caggcggggc tggcggcgga ggcggaggcg gaggtggtgg cggtgatgca
2161 gacggcggtt taggctcaaa tgtctcttta ggcaacacag tcggcacctc aactattgta
2221 ctggtttcgg gcgccgtttt tggtttgacc ggtctgagac gagtgcgatt tttttcgttt
2281 ctaatagctt ccaacaattg ttgtctgtcg tctaaaggtg cagcgggttg aggttccgtc
2341 ggcattggtg gagcgggcgg caattcagac atcgatggtg gtggtggtgg tggaggcgct
2401 ggaatgttag gcacgggaga aggtggtggc ggcggtgccg ccggtataat ttgttctggt
2461 ttagtttgtt cgcgcacgat tgtgggcacc ggcgcaggcg ccgctggctg cacaacggaa
2521 ggtcgtctgc ttcgaggcag cgcttggggt ggtggcaatt caatattata attggaatac
2581 aaatcgtaaa aatctgctat aagcattgta atttcgctat cgtttaccgt gccgatattt
2641 aacaaccgct caatgtaagc aattgtattg taaagagatt gtctcaagct cggatcgatc
2701 ccgcacgccg ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc
2761 gctgtcgtcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
2821 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
2881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
2941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
3001 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
3061 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
3121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
3181 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
3241 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
3301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
3361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
3421 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
3481 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
3541 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
3601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
3661 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
3721 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
3781 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
3841 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
3901 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
3961 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
4021 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
4081 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
4141 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
4201 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
4261 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
4321 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
4381 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
4441 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
4501 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
4561 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
4621 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
4681 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
4741 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
4801 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
4861 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
4921 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
4981 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
5041 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
5101 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
5161 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
5221 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
5281 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
5341 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
5401 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
5461 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
5521 gccgattcat taatgcag
