序列：
CDS complement(10392..11186)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=Kan
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
1 tcctcacgta gtgccaaagg aacgcgtgcg gtgaccacga cgggcttagc ctttgcctgc
61 gcttctagtg cttcgatggt ggcttgtgcc tgcgcttgct gcgcctgtag tgcctgttga
121 gcttcttgta gttgctgttc tagctgtgcc ttggttgcca tgctttaaga ctctagtagc
181 tttcctgcga tatgtcatgc gcatgcgtag caaacattgt cctgcaactc attcattatg
241 tgcagtgctc ctgttactag tcgtacatac tcatatttac ctagtctgca tgcagtgcat
301 gcacatgcag tcatgtcgtg ctaatgtgta aaacatgtac atgcagattg ctgggggtgc
361 agggggcgga gccaccctgt ccatgcgggg tgtggggctt gccccgccgg tacagacagt
421 gagcaccggg gcacctagtc gcggataccc cccctaggta tcggacacgt aaccctccca
481 tgtcgatgca aatctttaac attgagtacg ggtaagctgg cacgcatagc caagctaggc
541 ggccaccaaa caccactaaa aattaatagt ccctagacaa gacaaacccc cgtgcgagct
601 accaactcat atgcacgggg gccacataac ccgaaggggt ttcaattgac aaccatagca
661 ctagctaaga caacgggcac aacacccgca caaactcgca ctgcgcaacc ccgcacaaca
721 tcgggtctag gtaacactga aatagaagtg aacacctcta aggaaccgca ggtcaatgag
781 ggttctaagg tcactcgcgc tagggcgtgg cgtaggcaaa acgtcatgta caagatcacc
841 aatagtaagg ctctggcggg gtgccatagg tggcgcaggg acgaagctgt tgcggtgtcc
901 tggtcgtcta acggtgcttc gcagtttgag ggtctgcaaa actctcactc tcgctggggg
961 tcatctctgg ctgaattgga agtcatgggc gaacgccgca ttgagctggc tattgctact
1021 aagaatcact tggcggcggg tggcgcgctc atgatgtttg tgggcactgt tcgacacaac
1081 cgctcacagt catttgcgca ggttgaagcg ggtattaaga ctgcgtactc ttcgatggtg
1141 aaaacatctc agtggaagaa agaacgtgca cggtacgggg tggagcacac ctatagtgac
1201 tatgaggtca cagactcttg ggcgaacggt tggcacttgc accgcaacat gctgttgttc
1261 ttggatcgtc cactgtctga cgatgaactc aaggcgtttg aggattccat gttttcccgc
1321 tggtctgctg gtgtggttaa ggccggtatg gacgcgccac tgcgtgagca cggggtcaaa
1381 cttgatcagg tgtctacctg gggtggagac gctgcgaaaa tggcaaccta cctcgctaag
1441 ggcatgtctc aggaactgac tggctccgct actaaaaccg cgtctaaggg gtcgtacacg
1501 ccgtttcaga tgttggatat gttggccgat caaagcgacg ccggcgagga tatggacgct
1561 gttttggtgg ctcggtggcg tgagtatgag gttggttcta aaaacctgcg ttcgtcctgg
1621 tcacgtgggg ctaagcgtgc tttgggcatt gattacatag acgctgatgt acgtcgtgaa
1681 atggaagaag aactgtacaa gctcgccggt ctggaagcac cggaacgggt cgaatcaacc
1741 cgcgttgctg ttgctttggt gaagcccgat gattggaaac tgattcagtc tgatttcgcg
1801 gttaggcagt acgttctcga ttgcgtggat aaggctaagg acgtggccgc tgcgcaacgt
1861 gtcgctaatg aggtgctggc aagtctgggt gtggattcca ccccgtgcat gatcgttatg
1921 gatgatgtgg acttggacgc ggttctgcct actcatgggg acgctactaa gcgtgatctg
1981 aatgcggcgg tgttcgcggg taatgagcag actattcttc gcacccacta aaagcggcat
2041 aaaccccgtt cgatattttg tgcgatgaat ttatggtcaa tgtcgcgggg gcaaactatg
2101 atgggtcttg ttgttggcgt cccggagcgt aaaggatcta ggtgaagatc ctttttgata
2161 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccttgatg
2221 ataccgctgc cttactgggt gcattagcca gtctgaatga cctgtcacgg gataatccga
2281 agtggtcaga ctggaaaatc agagggcagg aactgctgaa cagcaaaaag tcagatagca
2341 ccacatagca gacccgccat aaaacgccct gagaagcccg tgacgggctt ttcttgtatt
2401 atgggtagtt tccttgcatg aatccataaa aggcgcctgt agtgccattt acccccattc
2461 actgccagag ccgtgagcgc agcgaactga atgtcacgaa aaagacagcg actcaggtgc
2521 ctgatggtcg gagacaaaag gaatattcag cgatttgccc gagcttgcga gggtgctact
2581 taagccttta gggttttaag gtctgttttg tagaggagca aacagcgttt gcgacatcct
2641 tttgtaatac tgcggaactg actaaagtag tgagttatac acagggctgg gatctattct
2701 ttttatcttt ttttattctt tctttattct ataaattata accacttgaa tataaacaaa
2761 aaaaacacac aaaggtctag cggaatttac agagggtcta gcagaattta caagttttcc
2821 agcaaaggtc tagcagaatt tacagatacc cacaactcaa aggaaaagga ctagtaatta
2881 tcattgacta gcccatctca attggtatag tgattaaaat cacctagacc aattgagatg
2941 tatgtctgaa ttagttgttt tcaaagcaaa tgaactagcg attagtcgct atgacttaac
3001 ggagcatgaa accaagctaa ttttatgctg tgtggcacta ctcaacccca cgattgaaaa
3061 ccctacaagg aaagaacgga cggtatcgtt cacttataac caatacgctc agatgatgaa
3121 catcagtagg gaaaatgctt atggtgtatt agctaaagca accagagagc tgatgacgag
3181 aactgtggaa atcaggaatc ctttggttaa aggctttgag attttccagt ggacaaacta
3241 tgccaagttc tcaagcgaaa aattagaatt agtttttagt gaagagatat tgccttatct
3301 tttccagtta aaaaaattca taaaatataa tctggaacat gttaagtctt ttgaaaacaa
3361 atactctatg aggatttatg agtggttatt aaaagaacta acacaaaaga aaactcacaa
3421 ggcaaatata gagattagcc ttgatgaatt taagttcatg ttaatgcttg aaaataacta
3481 ccatgagttt aaaaggctta accaatgggt tttgaaacca ataagtaaag atttaaacac
3541 ttacagcaat atgaaattgg tggttgataa gcgaggccgc ccgactgata cgttgatttt
3601 ccaagttgaa ctagatagac aaatggatct cgtaaccgaa cttgagaaca accagataaa
3661 aatgaatggt gacaaaatac caacaaccat tacatcagat tcctacctac ataacggact
3721 aagaaaaaca ctacacgatg ctttaactgc aaaaattcag ctcaccagtt ttgaggcaaa
3781 atttttgagt gacatgcaaa gtaagcatga tctcaatggt tcgttctcat ggctcacgca
3841 aaaacaacga accacactag agaacatact ggctaaatac ggaaggatct gaggttctta
3901 tggctcttgt atctatcagt gaagcatcaa gactaacaaa caaaagtaga acaactgttc
3961 accgggcccg tgggtggcta ggcaagttac agaactgatc ggataaaagc agagttatat
4021 ctgatgaatt gctattagca gtatcgttat cacagcacca acaaagtagt tcagccacag
4081 gaaaactttc caactgcgat tagcctgttc acaactggca tctgtaatgt tccaaaatcg
4141 tgcggcatta aatacgtaag ttagaatcgc aatcccgatg atccacgccg gattaggcaa
4201 agtagtgact aacacagcag ctagtaaata aagtactact gaaagccgaa tggctccacg
4261 cgccccaatt acagtggcaa ttgagctcag attagcttcc cggtctgcat taacatcctg
4321 tactgctcca aggatctgac tggccatgcc ccacaagaaa aaggatccca gtgctatcca
4381 catcgctgct gaaggagatg ttccagtgat cgttgcaccg attaatgcag gtgaagtgaa
4441 gtgagtagaa gatgttagag catcgataaa ggggcgttct ttaaaacgca atttcggtgc
4501 tgaataagca atcactgcta gcactgagag tgtcagccat aaagacgaca tccaggtgcc
4561 aaatatgaaa agaataacta ggaaaggaat tgttgagata gccgaggccc ataacagtgt
4621 gctgtgggaa cttttcggta gcacggcccc ctcgacgccg cctttgcggg gattacgcat
4681 atcagattcg taatcaaaaa catcgttgat accatacatg gcgatgttat acgggataag
4741 aaaaaatacg atgcctagcc aaaacagcca gtcaatctct cctgcattta ataggtaggc
4801 cagaccaaag gggtaggcgg tattgatcca gctaatgggg cgagatgaca atagaattag
4861 tcttattttt tccatcatga ctacggcttt tctggctcag attgcgtggt ggtggatcgc
4921 acccaatgag aactaggaga gtacctagat aaataaaggc cataaaaata tcgctatctt
4981 gctcattttg tgaaatatcg atgataggga tcaaaattta atgatcgtat gaggtctttt
5041 gagatggtgt cgttttaggc ggcaatggtt cgggcagtgg tcctgcactg gtatcggcat
5101 gtaaacgctt aataatattc tcggcagaaa ttaaacacat gggtattcct acacccggga
5161 cggtggtggc accggaatag aagaggttat tgaccttgcg ggagctattg cgccctctta
5221 agaaagcgga ctgtctgagg gtatgtgctg gacccagcgc actgcctacc catgaatggt
5281 agcggtgctc aaaatccgca gggccaatgg tgcgtttgac cacaattcgg tcagtgaggt
5341 cagggatgcc ggcttgcgta gcaatttgat tgattgcatg tgacgcgatt gtttccacgg
5401 atgctgaagc cgactgcata tacgcatcac cgtggccgat gctgctagag gccttggtcg
5461 gaattaaaac aaaaaggttt tcgtatccag caggtgcaac gccgtcttcg gacgttgaag
5521 gcttggagac ataaatggaa tttgatgcat tgtgggggcg ggtaagttga ggcccgtcga
5581 aaactacagc aaaatcatct gtccaatctt cactgaagaa aaggttgtga tggtcgagct
5641 ggggtaactc tccttttacg cccaggagga ttaataccgc tccaattcca ggattgcgat
5701 tggaccaata tcgttcggga taggttcgaa gttcccgggg aagcagatta ttttctgtat
5761 ggtgtaggtc gcctgctgaa accacaagat ccgcatctag attttgcact tttctgttgt
5821 gaagcaagct cacacctgtg gcgcttgtgt tgcccctcga tgaagcagtg ttgatggaaa
5881 tgacctcaga atcgagttga aactcaaccc cgttttccag cgctaactga tgcagagcgt
5941 taaccactgc agtaaaacca cctatagggt atttcactcc ctgcaccaaa tcggtatgac
6001 tcatcaagtg gtacatcgat ggggtagtag tgggtcggga agacaggaag actgctggat
6061 aggctagatt gacagctagc tcagtcctag gtataatgga tccgaatttc tactgttgta
6121 gatcaggcaa ccatagggca ggaaatttaa ataaaacgaa aggctcagtc gaaagactgg
6181 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
6241 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
6301 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
6361 ctacaaactc ttaagcttgc atgcctgcag gtcgacttag ttattgcggt tctggacaaa
6421 ttcaaagtat tcttcgttct taatgacgag attcagcttc ttgccttcct ggttgttctt
6481 gatgcgaccc aggagcatga gacccttcag accgatgtgg taggcgccgt ttgcatcagc
6541 gtcctgtggc atgttctttg gagcctggcg ggaatcgaag aagttgccgt tgacgtcggc
6601 cactggggag atgaggtaat ccagctcggt gccggtcttg gagttgcgca tctgcaggat
6661 ggtgttgagc acagaggtca gctttgcgaa gaacttcttg tcggattcgc cgcagattgc
6721 agccttgatg cactcgccgt ggccgtattc gatggagtaa tccttcagga gcttctccag
6781 ttccttggtt gggtacactt cgcgggtatc ccagttgtgg ttcttgtcgg agttgcggaa
6841 gttgatgagg cgggagccga aggatgcgat ggtccacttg cccttggctg ccttatcgcc
6901 gaagttcttg tagtcgaagg agaattcgaa gtagccctta tccaggttgt agcagatctt
6961 gtcgaacttg gagaagaact cctgggactt ggagacggat tcgtactttg ggtagagctg
7021 gttgacgaag ccggtcactg ggcagatctt agaggtgaag ccagctggca cgtagtagat
7081 gatgccggtc tgcttgccca tcttcttgaa ggtctcgaat ggggcggtca gctggtatgc
7141 acggaggacg ccgccggtct tatcgaattc gttgtccttg aacacgaggt agttcagctt
7201 ctcgatgagc atcttttcca gcttctggta gacctgcttc tccaccttga agcggccgcg
7261 cttgaagccg aagttcaggt cttcgaagac cacgattgcg ttgtactcga tcaccagctt
7321 agcgatttcg tggaccacct gggagaggta gccttccttc atttccttga tgttgttgat
7381 cttcttccaa tccttgcgag cggaatcgcg gtccttctcg atagcggcca gcttgtcgtg
7441 gtagttggtc ttcatgcgat cgttgccgat gatgttgaag gtgtcctgct tgatgatgtt
7501 gcccttgccg tcgaccaggg tgtagtaggc gaggtggcgt tcgccgcgat cgatggacag
7561 gatgtgcacg tcgttagcct tctccttcag gagcaggttg atttcatcgt tgaacttgtt
7621 ggcgccggag gacttgaagt tgatggtgat tgggcagtgg aagaagaact tgtcctcggt
7681 gaagcgctta tccttgatca ggtcgtactc gaacacggat tccttctttg ggttatcctt
7741 gttcttgttg gcgattgctt ccttggctgg gtgggtgatc ttctttggga tggactgctt
7801 gcggtagaac agctctgctt cgccgttgag cttgtagacc acatcctgca ggttgcgttc
7861 gtcgaagagt gccttccagt agagggtgtg caggtttggg cggcccttgg agtaagcgga
7921 gaagtccttg ttgtagatct ggaagaggta cagcttgccc tggttgacca cggaatcgat
7981 gtaggactcg gagatgtttt cgaaggtcag cttgtagccc tggttctcca cttcgcggta
8041 gaattcatcg atggagttgt agcgctgggt atcggagaag cggaagccga agtccttcca
8101 ctctgggtgc ttggagatgg actgcttgta gaaatcgatg aacttgcggc agtcttcgat
8161 gttgaactcg aacttttcgt agcccttctg tggggagccg ttcttggtgt gggtggagtg
8221 gttgcggatg cgcaggatat cttcggatgg gttgtagaac ttgatggact ttgcggagaa
8281 gaagaccttt gggagcatct tgttagcgcc tgggagcagc ttgtacacga tcttcttgta
8341 gccctcgccc ttgttttcct tgatggcctt atcgtcgaag atcttgttgt tcttcttgtt
8401 catcacgccc aggtagtact tatcgtcctt gatgaagagg atggcggtgt tatctggttc
8461 cttgttcttg tcccagccgt ttgccagggt ggagttctcg aagttgagct tgaacttttc
8521 atcggagtat ggcttctggg tgatgtagtt gcggatcttg ttgtacagtg ggacgatgtt
8581 agcgagttcg aagtagcact cttcgaacac caggtagaag tgctcatcct tgtcgaggat
8641 gttggccttg tcttcggact gggagatgtg gaagatcttc agcttgtgga gcaggttgtt
8701 ggtctgatcg agcaggtcct tgattgcctt cacatcgtcc tcagcggagg cctggagcag
8761 atccttcttg ccctggttct ggtacttgat ggagatctgg gccaggttat ccttgttctg
8821 tgcgatttcg tcgaagatca ttgggattgc agcgaagttt gcgaggatct cttcgaagcg
8881 gcactgctta tcgatgtcgc ggtgcttgtt gaactcttcg agagccagct tgatggtctc
8941 gagggacagg tacttagcct tttcggtctt cttggcgatc agctcctgtt ccttcttgga
9001 tgggttatcg aggttctttg gggcgatctg ctgggtgatg tattccagga ctgcggtgcc
9061 gatcacggag taatcgtcga agacctgctg ggagagatcg gtcagggact tgtcgttctt
9121 gaagtagatc ttggagagat ccagcttctg agccttcaga tcgtcgaaga gcagggagag
9181 ggtctccttg atggacttct cttccacggt cttgaaggct gcgatctgtt cgtagaagga
9241 ctgcatggtg gtgaccacat cggaatcgtc ctccagcttg tcgatgacga aggacttgga
9301 ttcggtgtcg gacaggatct gcttgaagag cacggacatc ttgtacttct tcagggtctt
9361 atcgttgatc tgctgggagt agaggttgat gtactcgttg atgcccttgc gcttggtgtt
9421 ttcgccgttg acgaacttgc cgccgatgat ggtgttgaac ttggtgatgc cggactggtt
9481 caggtagttg ttgaagttgg cgatctcgaa cacttcatcg agggagaaga cgcgctggtt
9541 cacttcagag gtcttgtaat cgatgtcgaa ggtgagctct tcggccaggt ccttcttgat
9601 ctgctcgtag ttgatagctt ctggggcctt atccttcagg gactcgtact ttgccttgtt
9661 ttcgaggaac tttggcaggt tatcgtcgac gatgcggtag atgatagagg ttgggatatc
9721 gttggaggag tacacgttct tgcggttttc gtggaagccc ttgaagtagg tggtccagcc
9781 cttgaaggac ttgatgatct ccagagcttc atcgatgtcg gtgatgtcgg agttggcctt
9841 gaagagctcg atgccgttat ccttggactg cttgagccac aggatgagat cggattcctg
9901 gcccttcttt gcgtcgatca ggttctggtt gaagaggttc ttgaacttct cggaatcctt
9961 gatgtattcg gagatctgct tcttgatggt atccttagcg gacttgaagt ccttctgcag
10021 gttatcgtca tcggacttct tgagcttgaa gtagacatcg gagtagttct ggagcaggtc
10081 ctcggagatg cacacggagg acaggatctc ttcgatgaag aactggtggt acttgtcgat
10141 gatctgcttt gccttcttgt aatccttagc gcgcttttcg tcatccagga tgaggccgcg
10201 tgccttgatg ttttcgaggg tcttgccttg ggggatcagc tcaaaacgga gggtcttgga
10261 cagggagtat ttattcacaa actcttggta gatggacatc gttcaagtcc tttccaattc
10321 cacacatggt accacacgat gattaattgt aaacagctca ggtcatgatt ccgcgaaccc
10381 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
10441 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
10501 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
10561 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
10621 ccatgggtca cgacgagatc ctcgccgtcg ggcatccgcg ccttgagcct ggcgaacagt
10681 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
10741 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
10801 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
10861 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
10921 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
10981 cacgatagcc gcgctgcctc gtcttggagt tcattcaggg caccggacag gtcggtcttg
11041 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
11101 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
11161 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat
11221 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
11281 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
11341 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
11401 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
11461 cgtttctgcg gactggcttt ctacgtgttc cgcttccggt gaccgcctcg atgatcgccg
11521 ggtgggcgtg gcccaaggag gatcatccag ccattcgggg tcgttcactg gttccccttt
11581 ctgatttctg gcatagaaga acccccgtga actgtgtggt tccgggggtt gctgattttt
11641 gcgagacttc tcgcgcaatt ccctagctta ggtgaaaaca ccatgaaaca ctagggaaac
11701 acccatgaaa cacccattag ggcagtaggg cggcttcttc gtctagggct tgcatttggg
11761 cggtgatctg gtctttagcg tgtgaaagtg tgtcgtaggt ggcgtgctca atgcactcga
11821 acgtcacgtc atttaccggg tcacggtggg caaagagaac tagtgggtta gacattgttt
11881 tcctcgttgt cggtggtggt gagcttttct agccgctcgg taaacgcggc gatcatgaac
11941 tcttggaggt tttcaccgtt ctgcatgcct gcgcgcttca tg
