pCDNA3.1(-)FLIPQTV3.0 100micro谷氨酰胺传感器哺乳质粒

  • 货号:KL-ZL1826-5ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL1826-5ug pCDNA3.1(-)FLIPQTV3.0 100micro谷氨酰胺传感器哺乳质粒 5ug

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货号 名称 规格
KL-ZL1826 pCDNA3.1(-)FLIPQTV3.0 100micro谷氨酰胺传感器哺乳质粒

5ug

原核抗性: 氨苄青霉素Amp 筛选标记: 遗传霉素G418 克隆菌株: 大肠杆菌DH5α 培养条件: 37℃
pCDNA3.1(-)FLIPQTV3.0 100micro谷氨酰胺传感器哺乳质粒

序列:
CDS complement(6531..7391)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7392..7496)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc
961 accacactgg actagtggat ccactcgaga tggcaggctc gaaattagtt gtcgcgacgg
1021 ataccgcctt cgttccgttc gaatttaaac aggccccaat ggtgagcaag ggcgaggaga
1081 ccacaatggg cgtaatcaag cccgacatga agatcaagct gaagatggag ggcaacgtga
1141 atggccacgc cttcgtgatc gagggcgagg gcgagggcaa gccctacgac ggcaccaaca
1201 ccatcaacct ggaggtgaag gagggagccc ccctgccctt ctcctacgac attctgacca
1261 ccgcgttcgc ctacggcaac agggccttca ccaagtaccc cgacgacatc cccaactact
1321 tcaagcagtc cttccccgag ggctactctt gggagcgcac catgaccttc gaggacaagg
1381 gcatcgtgaa ggtgaagtcc gacatctcca tggaggagga ctccttcatc tacgagatac
1441 acctcaaggg cgagaacttc ccccccaacg gccccgtgat gcagaagaag accaccggct
1501 gggacgcctc caccgagagg atgtacgtgc gcgacggcgt gctgaagggc gacgttaagc
1561 acaagctgct gctggagggc ggcggccacc accgcgttga cttcaagacc atctacaggg
1621 ccaagaaggc ggtgaagctg cccgactatc actttgtgga ccaccgcatc gagatcctga
1681 accacgacaa ggactacaac aaggtgaccg tttacgagag cgccgtaccc gcaactccac
1741 cgacggcatg gacgagctgt acaaggtgag cgccaaatat gtgggctttg acgtcgatct
1801 gtgggctgcc atcgctaaag agctgaagct ggattacgaa ctgaagccga tggatttcag
1861 tgggatcatt ccggcactgc aaaccaaaaa cgtcgatctg gcgctggcgg gcattaccat
1921 caccgacgag cgtaaaaaag cgatcgattt ctctgacggc tactacaaaa gcggcctgtt
1981 agtgatggtg aaagctaaca ataacgatgt gaaaagcgtg aaagatctcg acgggaaagt
2041 ggttgctgtg aagagcggta ctggctccgt tgattacgcg aaagcaaaca tcaaaactaa
2101 agatctgcgt cagttcccga acatcgataa cgcctatatg gaactgggca ccaaccgcgc
2161 agacgccgtt ctgcacaaca cgccaaacat tctgtacttc atcaaaaccg ccggtaacgg
2221 tcagttcaaa gcggtaggtg actctctgga agcgcagcaa tacggtattg cgttcccgaa
2281 aggtagcgac gagctgcgtg acaaagtcaa cggcgcgttg aaaaccctgc gcgagaacgg
2341 aacttacaac gaaatctaca aaaaatggtt cggtactgaa ccgaaaggcg ctgagctgtt
2401 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
2461 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
2521 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct
2581 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
2641 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
2701 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
2761 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
2821 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
2881 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
2941 cggcgacggc cccgtgctgc tgcccgacaa tcactacctg agctaccagt ccgccctgag
3001 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
3061 gatcactctc ggcatggacg agctgtacaa gtaaaagctt aagtttaaac cgctgatcag
3121 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
3181 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
3241 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
3301 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
3361 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
3421 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
3481 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
3541 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
3601 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
3661 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
3721 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
3781 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
3841 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
3901 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
3961 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
4021 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
4081 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
4141 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
4201 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
4261 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
4321 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
4381 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
4441 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
4501 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
4561 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
4621 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
4681 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
4741 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
4801 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
4861 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
4921 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
4981 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
5041 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
5101 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
5161 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
5221 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
5281 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
5341 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
5401 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
5461 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
5521 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
5581 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
5641 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
5701 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
5761 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
5821 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
5881 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
5941 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
6001 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
6061 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
6121 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
6181 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
6241 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
6301 gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
6361 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
6421 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
6481 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
6541 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
6601 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
6661 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
6721 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
6781 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
6841 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
6901 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
6961 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
7021 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
7081 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
7141 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
7201 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
7261 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
7321 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
7381 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
7441 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
7501 tttccccgaa aagtgccacc tgacgtc

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