pXT7-Cas9 爪蟾基因编辑Cas9表达

  • 货号:KL-ZL1823-5ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL1823-5ug pXT7-Cas9 爪蟾基因编辑Cas9表达 5ug

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货号 名称 规格
KL-ZL1823 pXT7-Cas9 爪蟾基因编辑Cas9表达

5ug

原核抗性: 氨苄青霉素Amp 克隆菌株: 大肠杆菌Stbl3 培养条件: 37℃
pXT7-Cas9 爪蟾基因编辑Cas9表达载体

序列:
CDS complement(6176..7036)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7037..7141)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tagcgtatca gagtctcgtc cgtcggtcgt atgacggtga aacctctgac acatgcagct
61 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
121 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
181 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
241 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
301 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
361 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattggattt
421 aggtgacact atagaatacg aattaattcg agctcgcccg gggatcctct agagtcgacc
481 tgcagggggg gggggggggg gttttttttt tttttttttt tttttttttt tttttagaat
541 gtgaagaaac tttcttttta ttaggagcag atacgaatgg ctacattttg ggggacaaca
601 ttttgtaaag tgtaagttgg tattatgtag cttagagact ccattcgggt gttcttgagg
661 ctggtttagt ggtaaccaga tcctagtcag tcactagatt atttcttttt cttagcttga
721 ccagctttct tagtagcagc aggacgcttg tctccaccga gctgagagag gtcgattctt
781 gtttcataga gccccgtaat tgactgatga atcagtgtgg cgtccaggac ctcctttgta
841 gaggtgtacc gctttctgtc tatggtggtg tcgaagtact tgaaggctgc aggcgcgccc
901 aagttggtca gagtaaacaa gtggataatg ttttctgcct gctccctgat gggcttatcc
961 ctgtgcttat tgtaagcaga aagcacctta tcgaggttag cgtcggcgag gatcactctt
1021 ttggagaatt cgcttatttg ctcgatgatc tcatcaaggt agtgtttgtg ttgttccacg
1081 aacagctgct tctgctcatt atcttcggga gaccctttga gcttttcata gtggctggcc
1141 agatacaaga aattaacgta tttagagggc agtgccagct cgttaccttt ctgcagctcg
1201 cccgcactag cgagcattcg tttccggccg ttttcaagct caaagagaga gtacttggga
1261 agcttaatga tgaggtcttt tttgacctct ttatatcctt tcgcctcgag aaagtcgatg
1321 gggttttttt cgaagcttga tcgctccatg attgtgatgc ccagcagttc cttgacgctt
1381 ttgagttttt tagacttccc tttctccact ttggccacaa ccagtacact gtaagcgact
1441 gtaggagaat cgaatccgcc gtatttcttg gggtcccaat cttttttgcg tgcgatcagc
1501 ttgtcgctgt tccttttcgg gaggatactt tccttggaga agcctccggt ctgtacttcg
1561 gtctttttaa cgatgttcac ctgcggcatg gacaggacct tccggactgt cgcgaaatcc
1621 ctacccttgt cccacacgat ttctcctgtt tctccgtttg tttcgataag tggtcgcttc
1681 cgaatctctc cattggccag tgtaatctcg gtcttgaaaa aattcataat attgctgtaa
1741 aagaagtact tagcggtggc cttgcctatt tcctgctcag actttgcgat cattttccta
1801 acatcgtaca ctttatagtc tccgtaaaca aattcagatt caagcttggg atattttttg
1861 ataagtgcag tgcctaccac tgcattcagg taggcatcat gcgcatggtg gtaattgttg
1921 atctctctca ccttataaaa ctgaaagtcc tttctgaaat ctgagaccag cttagacttc
1981 agagtaataa ctttcacctc tcgaatcagt ttgtcatttt catcgtactt ggtgttcatg
2041 cgtgaatcga gaatttgggc cacgtgcttg gtgatctggc gtgtctcaac aagctgcctt
2101 ttgatgaagc ctgctttatc caactcagac aggccacctc gttcagcctt agtcagatta
2161 tcgaacttcc gttgtgtgat cagtttggcg ttcagcagct gccgccaata atttttcatt
2221 ttcttgacaa cttcttctga ggggacgtta tcactcttcc ctctattttt atcggatctt
2281 gtcaacactt tattatcaat agaatcatct ttgagaaaag actggggcac gatatgatcc
2341 acgtcgtagt cggagagccg attgatgtcc agttcctgat ccacgtacat gtccctgccg
2401 ttctgcaggt agtacaggta gagcttctca ttctgaagct gggtgttttc aactgggtgt
2461 tccttaagga tttgggaccc cagttctttt ataccctctt caatcctctt catcctttcc
2521 ctactgttct tctgtccctt ctgggtagtt tggttctctc gggccatctc gataacgata
2581 ttctcgggct tatgccttcc cattactttg acgagttcat ccacgacctt aacggtctgc
2641 agtattccct ttttgatagc tgggctacct gcaagattag cgatgtgctc gtgaagactg
2701 tccccctggc cagaaacttg tgctttctgg atgtcctcct taaaggtgag agagtcatca
2761 tggatcaact gcatgaagtt ccggttggca aatccatcgg acttaagaaa atccaggatt
2821 gtctttccac tctgcttgtc tcggatccca ttgatcagtt ttcttgacag ccgcccccat
2881 cctgtatatc ggcgcctctt gagctgtttc atgactttgt cgtcgaagag atgagcgtaa
2941 gttttcaagc gttcttcaat catctcccta tcttcaaaca acgtaagggt gaggacaatg
3001 tcctcaagaa tgtcctcgtt ctcctcattg tccaggaagt ccttgtcttt aatgattttc
3061 aggagatcgt gatacgttcc cagggatgcg ttgaagcgat cctccactcc gctgatttca
3121 acagagtcga aacattcaat ctttttgaaa tagtcttctt tgagctgttt cacggtaact
3181 ttccggttcg tcttgaagag gaggtccacg atagctttct tctgctctcc agacaggaat
3241 gctggctttc tcatcccttc tgtgacgtat ttgaccttgg tgagctcgtt ataaactgtg
3301 aagtactcgt acagcagaga gtgtttagga agcacctttt cgttaggcag atttttatca
3361 aagttagtca tcctttcgat gaaggactgg gcagaggccc ccttatccac gacttcctcg
3421 aagttccagg gagtgatggt ctcttctgat ttgcgagtca tccacgcgaa tctggaattt
3481 ccccgggcga gggggcctac atagtagggt atccgaaatg tgaggatttt ctcaatcttt
3541 tccctgttat ctttcaaaaa ggggtagaaa tcctcttgcc gcctgagtat agcgtgcagt
3601 tcgcccaggt gaatctggtg ggggatgctt ccattgtcga aagtgcgctg tttgcgcaac
3661 agatcttctc tgttaagctt taccagcagc tcctcggtgc cgtccatttt ttccaagatg
3721 ggcttaataa atttgtaaaa ttcctcctgg cttgctccgc cgtcaatgta tccggcgtag
3781 ccatttttag actgatcgaa gaaaatttcc ttgtacttct caggcagttg ctgtctgaca
3841 agggccttca gcaaagtcaa gtcttggtgg tgctcatcat agcgcttgat catactagcg
3901 ctcagcggag ctttggtgat ctccgtgttc actcgcagaa tatcactcag cagaatggcg
3961 tctgacaggt tctttgccgc caaaaaaagg tctgcgtact ggtcgccgat ctgggccagc
4021 agattgtcga gatcatcatc gtaggtgtct ttgctcagtt gaagcttggc atcttcggcc
4081 aggtcgaagt tagatttaaa gttgggggtc agcccgagtg acagggcgat aagattacca
4141 aacaggccgt tcttcttctc cccagggagc tgtgcgatga ggttttcgag ccgccgggat
4201 ttggacagcc tagcgctcag gattgctttg gcgtcaactc cggatgcgtt gatcgggttc
4261 tcttcgaaaa gctgattgta agtctgaacc agttggataa agagtttgtc gacatcgctg
4321 ttgtctgggt tcaggtcccc ctcgatgagg aagtgtcccc gaaatttgat catatgcgcc
4381 agcgcgagat agatcaaccg caagtcagcc ttatcagtac tgtctacaag cttcttcctc
4441 agatgatata tggttgggta cttttcatgg tacgccacct cgtccacgat attgccaaag
4501 attgggtggc gctcgtgctt tttatcctcc tccaccaaaa aggactcctc cagcctatgg
4561 aagaaagagt catccacctt agccatctca ttactaaaga tctcctgcag gtagcagatc
4621 cgattctttc tgcgggtata tctgcgccgt gctgttcttt tgagccgcgt ggcttcggcc
4681 gtctccccgg agtcgaacag gagggcgcca atgaggttct tctttatgct gtggcgatcg
4741 gtattgccca gaactttgaa ttttttgctc ggcaccttgt actcgtccgt aatgacggcc
4801 cagccgacgc tgtttgtgcc gatatcgagc ccaatggagt acttcttgtc catggctgct
4861 gggacaccgt ggataccgac cttccgcttc ttctttgggg ccatggtggc ggtaccgatc
4921 tgccaaagtt gagcgtttat tctgagcttc tgcaaaaaga acaagcaagc ttgtctccct
4981 atagtgagtc gtattagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
5041 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
5101 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
5161 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
5221 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
5281 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
5341 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
5401 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
5461 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
5521 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
5581 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
5641 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
5701 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
5761 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
5821 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
5881 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
5941 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
6001 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
6061 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
6121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
6181 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
6241 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
6301 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
6361 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
6421 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
6481 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
6541 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
6601 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
6661 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
6721 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
6781 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
6841 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
6901 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
6961 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
7021 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
7081 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
7141 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
7201 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
7261 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
7321 ggagcagaca agcccgtcag ggcgcgtcag cggt

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