pCDNA3.1-HA-UB-KO人源基因哺乳表达质粒

  • 货号:KL-ZL1817-5ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL1817-5ug pCDNA3.1-HA-UB-KO人源基因哺乳表达质粒 5ug

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货号 名称 规格
KL-ZL1817 pCDNA3.1-HA-UB-KO人源基因哺乳表达质粒

5ug

原核抗性: 氨苄青霉素Amp 筛选标记: 遗传霉素G418 克隆菌株: 大肠杆菌DH5α 培养条件: 37℃
pCDNA3.1-HA-UB-KO是一个人源泛素基因哺乳细胞表达质粒

序列:
CDS complement(4661..5521)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5522..5626)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
901 cgccatgtac ccttatgacg tccccgatta cgccagcctg tctagaggca tgcagatctt
961 cgtgaggacc ctgactggta ggaccatcac tctcgaagtg gagccgagtg acaccattga
1021 gaatgtcagg gcaaggatcc aagacaggga aggcatccct cctgaccagc agaggttgat
1081 ctttgctggg aggcagctgg aagatggacg caccctgtct gactacaaca tccagaggga
1141 gtccaccctg cacctggtcc tccgtctcag aggtgggtga gaattctgca gatatccagc
1201 acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
1261 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
1321 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
1381 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
1441 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
1501 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
1561 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
1621 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
1681 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
1741 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
1801 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
1861 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
1921 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
1981 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
2041 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
2101 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
2161 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
2221 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
2281 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
2341 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
2401 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
2461 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
2521 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
2581 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
2641 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
2701 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
2761 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
2821 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
2881 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
2941 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
3001 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
3061 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
3121 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
3181 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
3241 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
3301 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
3361 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
3421 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
3481 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
3541 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
3601 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
3661 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
3721 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
3781 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
3841 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
3901 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
3961 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
4021 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
4081 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
4141 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
4201 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
4261 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
4321 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
4381 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
4441 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
4501 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
4561 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
4621 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
4681 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
4741 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
4801 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
4861 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
4921 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
4981 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
5041 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
5101 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
5161 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
5221 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
5281 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
5341 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
5401 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
5461 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
5521 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
5581 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
5641 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt

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