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pT7-His-hCD40LG人源基因大肠表达质粒

  • 货号：KL-ZL1814-5ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL1814-5ug pT7-His-hCD40LG人源基因大肠表达质粒 5ug

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》产品信息

货号 名称 规格
KL-ZL1814 pT7-His-hCD40LG人源基因大肠表达质粒

5ug

》产品描述

别名： CD154; gp39 原核抗性： 卡那霉素Kan 克隆菌株： 大肠杆菌DH5α 培养条件： 37℃

》产品简介

pT7-His-hCD40LG是一个人源基因大肠表达质粒。

》产品图谱及序列


序列：
CDS complement(4739..5821)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter complement(5822..5899)
/gene="lacI"
/note="lacI promoter"
ORIGIN
1 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
61 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
121 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
181 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
241 atcatcatca tcatcacagc agcggccgcg gatccatgat cgaaacatac aaccaaactt
301 ctccccgatc tgcggccact ggactgccca tcagcatgaa aatttttatg tatttactta
361 ctgtttttct tatcacccag atgattgggt cagcactttt tgctgtgtat cttcatagaa
421 ggttggacaa gatagaagat gaaaggaatc ttcatgaaga ttttgtattc atgaaaacga
481 tacagagatg caacacagga gaaagatcct tatccttact gaactgtgag gagattaaaa
541 gccagtttga aggctttgtg aaggatataa tgttaaacaa agaggagacg aagaaagaaa
601 acagctttga aatgcaaaaa ggtgatcaga atcctcaaat tgcggcacat gtcataagtg
661 aggccagcag taaaacaaca tctgtgttac agtgggctga aaaaggatac tacaccatga
721 gcaacaactt ggtaaccctg gaaaatggga aacagctgac cgttaaaaga caaggactct
781 attatatcta tgcccaagtc accttctgtt ccaatcggga agcttcgagt caagctccat
841 ttatagccag cctctgccta aagtcccccg gtagattcga gagaatctta ctcagagctg
901 caaataccca cagttccgcc aaaccttgcg ggcaacaatc cattcacttg ggaggagtat
961 ttgaattgca accaggtgct tcggtgtttg tcaatgtgac tgatccaagc caagtgagcc
1021 atggcactgg cttcacgtcc tttggcttac tcaaactctg actcgagcac caccaccacc
1081 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
1141 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
1201 tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag
1261 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
1321 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
1381 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
1441 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
1501 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
1561 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
1621 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
1681 gtttacaatt tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
1741 tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga
1801 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
1861 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
1921 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
1981 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
2041 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
2101 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
2161 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
2221 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
2281 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
2341 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
2401 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
2461 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
2521 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt
2581 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
2641 ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
2701 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
2761 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
2821 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
2881 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
2941 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
3001 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
3061 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
3121 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
3181 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
3241 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
3301 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
3361 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
3421 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
3481 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
3541 cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
3601 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
3661 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
3721 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
3781 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
3841 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
3901 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
3961 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
4021 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
4081 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
4141 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
4201 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
4261 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
4321 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
4381 gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
4441 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
4501 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
4561 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
4621 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
4681 catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
4741 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
4801 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
4861 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
4921 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
4981 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
5041 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
5101 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
5161 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
5221 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
5281 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
5341 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
5401 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
5461 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
5521 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
5581 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
5641 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
5701 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
5761 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
5821 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
5881 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
5941 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
6001 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
6061 caagcgc

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