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pLacZi酿酒酵母单杂交质粒

货号：KL-ZL1808-5ug

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL1808-5ug	pLacZi酿酒酵母单杂交质粒	5ug		立即咨询	+ -

产品介绍
相关产品

》产品信息

货号	名称	规格
KL-ZL1808	pLacZi酿酒酵母单杂交质粒	5ug

》产品描述

启动子： CYC1 原核抗性：氨苄青霉素Amp 筛选标记： URA3 克隆菌株：大肠杆菌DH5α 培养条件： 37℃

》产品简介

pLacZi是一个酿酒酵母单杂交质粒。

》产品图谱及序列

序列：

CDS complement(5848..6651)

/codon_start=1

/gene="S. cerevisiae URA3"

/product="orotidine-5'-phosphate decarboxylase, required

for uracil biosynthesis"

/label=URA3

/note="yeast auxotrophic marker, counterselectable with

5-fluoroorotic acid (5-FOA)"

/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL

ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ

YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE

YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD

VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"

promoter complement(6652..6867)

/gene="S. cerevisiae URA3"

/label=URA3 promoter

ORIGIN

1 aagcttgaat tcgagctcgg tacccgggga tctgtcgacc tcgaggcatg tgctctgtat

61 gtatataaaa ctcttgtttt cttcttttct ctaaatattc tttccttata cattaggtcc

121 tttgtagcat aaattactat acttctatag acacgcaaac acaaatacac acactaaatt

181 aataatgacc ggatccggag cttggctgtt gcccgtctca ctggtgaaaa gaaaaaccac

241 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct

301 ggcacgacag gtttcccgac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg

361 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga

421 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga

481 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc

541 gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga

601 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg

661 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg

721 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt

781 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct

841 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa

901 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg

961 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac

1021 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga

1081 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa

1141 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac

1201 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat

1261 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca

1321 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct

1381 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct

1441 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac

1501 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag

1561 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc

1621 gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc

1681 tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga

1741 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa

1801 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga

1861 atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg

1921 tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa

1981 atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa

2041 cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct

2101 gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga

2161 agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc

2221 gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa

2281 acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac

2341 agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg

2401 gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat

2461 cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca

2521 atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct

2581 gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga

2641 agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca

2701 ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac

2761 gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg

2821 gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc

2881 gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg

2941 gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg

3001 ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct

3061 gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt

3121 caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca

3181 cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga

3241 ctcctggagc ccgtcagtat cggcggaatt acagctgagc gccggtcgct accattacca

3301 gttggtctgg tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt

3361 aaggaaatcc attatgtact atttaaaaaa cacaaacttt tggatgttcg gtttattctt

3421 tttcttttac ttttttatca tgggagccta cttcccgttt ttcccgattt ggctacatga

3481 catcaaccat atcagcaaaa gtgatacggg tattattttt gccgctattt ctctgttctc

3541 gctattattc caaccgctgt ttggtctgct ttctgacaaa ctcggcctcg actctagcga

3601 attaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc

3661 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct

3721 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc

3781 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt

3841 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag

3901 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca

3961 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt

4021 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc

4081 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct

4141 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg

4201 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca

4261 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact

4321 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta

4381 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta

4441 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct

4501 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt

4561 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga

4621 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca

4681 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat

4741 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg

4801 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt

4861 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag

4921 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc

4981 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag

5041 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca

5101 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa

5161 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga

5221 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata

5281 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca

5341 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg

5401 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg

5461 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg

5521 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag

5581 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac

5641 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca

5701 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag

5761 tgccacctgg gtaataactc gatataatta aattgaagct ctaatttgtg agtttagtat

5821 acatgcattt acttataata cagtttttta gttttgctgg ccgcatcttc tcaaatatgc

5881 ttcccagcct gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa

5941 tagtcctctt ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct

6001 atactgttga cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa

6061 ccaatcgtaa ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa

6121 atctttgtcg ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga

6181 gcccttgcat gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc

6241 tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat

6301 gtctgcccat tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc

6361 aatgtcagca aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt

6421 tagcggctta actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag

6481 taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac

6541 atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc

6601 aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct

6661 tcgtttcctg caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt

6721 ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc

6781 ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa

6841 agaataaaaa aaaaatgatg aattgaa

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