pECMV-3×Flag-Nrf2-m小鼠基因质粒

  • 货号:KL-ZL1631-2ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL1631-2ug pECMV-3×Flag-Nrf2-m小鼠基因质粒 2ug

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货号 名称 规格
KL-ZL1631 pECMV-3×Flag-Nrf2-m小鼠基因质粒

2ug

启动子: CMV promoter 复制子: pUC ori,F1 ori,SV40 ori 终止子: bGH poly(A) signal 质粒大小: 7247bp 质粒标签: N-3xFLAG 原核抗性: 氨苄青霉素Amp 筛选标记: 新霉素Neo/G418 克隆菌株: 大肠杆菌Stbl3 培养条件: 37℃,有氧,LB 表达宿主: 哺乳细胞293T
pECMV-3×FLAG-Nrf2-m是一个哺乳细胞过表达质粒,CMV启动子驱动3×FLAG标签和Nrf2小鼠源基因融合表达。

序列:
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac
961 atcgactaca aggatgacga tgacaagctt ggtaccgcca ccatgatgga cttggagttg
1021 ccaccgccag gactacagtc ccagcaggac atggatttga ttgacatcct ttggaggcaa
1081 gacatagatc ttggagtaag tcgagaagtg tttgacttta gtcagcgaca gaaggactat
1141 gagctggaaa aacagaaaaa actcgaaaag gaaagacaag agcaactcca gaaggaacag
1201 gagaaggcct tttttgctca gtttcaactg gatgaagaaa caggagaatt cctcccaatt
1261 cagccggccc agcacatcca gacagacacc agtggatccg ccagctactc ccaggttgcc
1321 cacattccca aacaagatgc cttgtacttt gaagactgta tgcagctttt ggcagagaca
1381 ttcccatttg tagatgacca tgagtcgctt gccctggata tccccagcca cgctgaaagt
1441 tcagtcttca ctgcccctca tcaggcccag tccctcaata gctctctgga ggcagccatg
1501 actgatttaa gcagcataga gcaggacatg gagcaagttt ggcaggagct attttccatt
1561 cccgaattac agtgtcttaa taccgaaaac aagcagctgg ctgatactac cgctgttccc
1621 agcccagaag ccacactgac agaaatggac agcaattacc atttttactc atcgatctcc
1681 tcgctggaaa aagaagtggg caactgtggt ccacatttcc ttcatggttt tgaggattct
1741 ttcagcagca tcctctccac tgatgatgcc agccagctga cctccttaga ctcaaatccc
1801 accttaaaca cagattttgg cgatgaattt tattctgctt tcatagcaga gcccagtgac
1861 ggtggcagca tgccttcctc cgctgccatc agtcagtcac tctctgaact cctggacggg
1921 actattgaag gctgtgacct gtcactgtgt aaagctttca acccgaagca cgctgaaggc
1981 acaatggaat tcaatgactc tgactctggc atttcactga acacgagtcc cagccgagcg
2041 tccccagagc actccgtgga gtcttccatt tacggagacc caccgcctgg gttcagtgac
2101 tcggaaatgg aggagctaga tagtgcccct ggaagtgtca aacagaacgg ccctaaagca
2161 cagccagcac attctcctgg agacacagta cagcctctgt caccagctca agggcacagt
2221 gctcctatgc gtgaatccca atgtgaaaat acaacaaaaa aagaagttcc cgtgagtcct
2281 ggtcatcaaa aagccccatt cacaaaagac aaacattcaa gccgcttaga ggctcatctc
2341 acacgagatg agcttagggc aaaagctctc catattccat tccctgtcga aaaaatcatt
2401 aacctccctg ttgatgactt caatgaaatg atgtccaagg agcaattcaa tgaagctcag
2461 ctcgcattga tccgagatat acgcaggaga ggtaagaata aagtcgccgc ccagaactgt
2521 aggaaaagga agctggagaa cattgtcgag ctggagcaag acttgggcca cttaaaagac
2581 gagagagaaa aactactcag agaaaaggga gaaaacgaca gaaacctcca tctactgaaa
2641 aggcggctca gcaccttgta tcttgaagtc ttcagcatgt tacgtgatga ggatggaaag
2701 ccttactctc ccagtgaata ctctctgcag caaaccagag atggcaatgt gttccttgtt
2761 cccaaaagca agaagccaga tacaaagaaa aactaggcgg ccgctcgagt ctagagggcc
2821 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
2881 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
2941 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
3001 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
3061 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
3121 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
3181 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
3241 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
3301 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
3361 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
3421 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
3481 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
3541 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
3601 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
3661 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
3721 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
3781 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
3841 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
3901 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
3961 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
4021 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
4081 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
4141 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
4201 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
4261 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
4321 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
4381 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
4441 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
4501 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
4561 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
4621 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
4681 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
4741 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
4801 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
4861 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
4921 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
4981 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
5041 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
5101 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
5161 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
5221 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
5281 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
5341 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
5401 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
5461 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
5521 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
5581 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
5641 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
5701 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
5761 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
5821 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
5881 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
5941 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
6001 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc
6061 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
6121 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
6181 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
6241 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
6301 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
6361 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
6421 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
6481 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
6541 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
6601 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
6661 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
6721 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
6781 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
6841 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
6901 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
6961 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
7021 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
7081 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
7141 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
7201 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc

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