质粒大小： 10640bp
原核抗性： 卡那霉素Kan
筛选标记： 潮霉素Hyg
克隆菌株： 大肠杆菌DB3.1
培养条件： 37℃，有氧，LB
pCXGFP-P质粒是一个植物启动子荧光检测载体，通过GFP的荧光强度来检测启动子活性高低。使用XcmI酶切空载体得到T载体，可连接带A的PCR产物，并在ccdb致死基因的帮助下，基因克隆的阳性率得以大大提高。
序列：
ORIGIN
1 gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
61 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
121 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
181 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
241 ttgccaaatg tttgaacgat cggggaaatt cgagctctca tttgtatagt tcatccatgc
301 catgtgtaat cccagcagct gttacaaact caagaaggac catgtggtct ctcttttcgt
361 tgggatcttt cgaaagggca gattgtgtgg acaggtaatg gttgtctggt aaaaggacag
421 ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg cttccatctt
481 caatgttgtg tctaattttg aagttaactt tgattccatt cttttgtttg tctgccatga
541 tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg tccaagaatg tttccatctt
601 ctttaaaatc aatacctttt aactcgattc tattaacaag ggtatcacct tcaaacttga
661 cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat
721 aaccttcggg catggcactc ttgaaaaagt catgccgttt catatgatct gggtatcttg
781 aaaagcatag aacaccataa cccatagtag tgacaagtgt tggccatgga acaggtagtt
841 ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca ccttcaccct
901 ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca agaattggga
961 caactccagt gaaaagttct tctcctttac ccatggatcc ccaatacttg tatggggctt
1021 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
1081 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
1141 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
1201 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
1261 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
1321 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
1381 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
1441 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
1501 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
1561 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
1621 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
1681 gcaggtcgac ccatacaagt attggggatc ctctagagtc gacctgcagg catgcaagct
1741 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
1801 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
1861 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
1921 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
1981 gggtaaacct aagagaaaag agcgtttatt agaataacgg atatttaaaa gggcgtgaaa
2041 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
2101 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
2161 gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
2221 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
2281 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
2341 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
2401 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
2461 caggatgctt gaccacctag ccctggcgac gttgtgacag tgaccaggct agaccgcctg
2521 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
2581 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
2641 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
2701 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
2761 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
2821 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
2881 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
2941 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
3001 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
3061 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
3121 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
3181 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
3241 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
3301 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
3361 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
3421 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
3481 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
3541 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
3601 gcagccaagc ccttacgaca tatgggcaac cgccgacctg gtggagctgg ttaagcagcg
3661 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
3721 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
3781 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
3841 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
3901 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
3961 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
4021 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
4081 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
4141 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
4201 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
4261 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
4321 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
4381 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
4441 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
4501 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
4561 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
4621 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
4681 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
4741 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
4801 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
4861 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
4921 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
4981 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
5041 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
5101 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
5161 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
5221 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
5281 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
5341 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
5401 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
5461 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
5521 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
5581 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
5641 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
5701 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
5761 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
5821 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
5881 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
5941 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
6001 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
6061 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
6121 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
6181 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
6241 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
6301 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
6361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
6421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
6481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
6541 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
6601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
6661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
6721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
6781 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
6841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
6901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
6961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
7021 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
7081 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
7141 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
7201 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
7261 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
7321 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
7381 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
7441 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
7501 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
7561 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
7621 atagcatcat gtccttttcc cgttccacat cataggttgt ccctttatac cggctgtccg
7681 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
7741 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
7801 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
7861 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
7921 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
7981 ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca
8041 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
8101 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
8161 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
8221 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
8281 caacttaata acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg
8341 gggatctgga ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag
8401 tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac
8461 cctataggaa ccctaattcc cttatctggg aactactcac acattattat ggagaaactc
8521 gagcttgtcg atcgacagat ccggtcggca tctactctat ttctttgccc tcggacgagt
8581 gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc
8641 cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc
8701 gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata
8761 gagttggtca agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc
8821 cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca
8881 gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat
8941 gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac
9001 gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc
9061 gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc
9121 aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg
9181 gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac
9241 cggttgtaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc
9301 acggcgggag atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg
9361 aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc
9421 ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg
9481 agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag
9541 aaacttctcg acagacgtcg cggtgagttc aggctttttc atatctcatt gccccccgga
9601 tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct
9661 ctccaaatga aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt
9721 gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt
9781 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
9841 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
9901 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
9961 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
10021 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat
10081 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
10141 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
10201 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
10261 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
10321 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
10381 gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg
10441 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
10501 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
10561 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
10621 acagctatga ccatgattac
