启动子： CaMV35S promoter
终止子： NOS terminator
质粒大小： 11277bp
原核抗性： 卡那霉素Kan
筛选标记： 潮霉素Hyg
克隆菌株： 大肠杆菌DB3.1
培养条件： 37℃，有氧，LB
pCX-DG质粒是一个植物过表达质粒，35S启动子驱动GFP绿色荧光蛋白和目的基因融合表达，在ccdb致死基因的作用下，克隆基因变得更加容易，阳性率高。
序列：
ORIGIN
1 gaattcgagc tcggtaccca ctggattttg gttttaggaa ttagaaattt tattgataga
61 agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa
121 accctatagg aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac
181 tcgaaattcg agctccaccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc
241 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
301 ctccgctcat gatccgggga attaattccg atcctatctg tcacttcatc aaaaggacag
361 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcattc
421 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
481 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
541 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
601 gttcatttca tttggagagg acacgctgaa atcaccagtc tctctctaca agatcctcta
661 gcgctaccgg tcgccaccat gggtaaagga gaagaacttt tcactggagt tgtcccaatt
721 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
781 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
841 gttccatggc caacacttgt cactactatg ggttatggtg ttctatgctt ttcaagatac
901 ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
961 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
1021 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
1081 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
1141 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
1201 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
1261 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
1321 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
1381 gaactataca agtccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt
1441 accgcgggcc cgggatcccc aatacttgta tggggcttac taaaagccag ataacagtat
1501 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
1561 atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct
1621 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
1681 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
1741 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg
1801 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
1861 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
1921 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
1981 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
2041 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
2101 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgaccc atacaagtat
2161 tggggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
2221 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
2281 attgttgttg ttgtgtgaat tacaggtgac cagctcgaat ttccccgatc gttcaaacat
2341 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
2401 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
2461 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
2521 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
2581 ggaattaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
2641 gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
2701 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
2761 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
2821 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
2881 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
2941 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
3001 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
3061 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctagccc
3121 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
3181 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
3241 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
3301 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
3361 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
3421 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
3481 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
3541 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
3601 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
3661 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
3721 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
3781 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
3841 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
3901 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
3961 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
4021 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
4081 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
4141 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
4201 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
4261 gggcaaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
4321 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
4381 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
4441 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
4501 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
4561 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
4621 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
4681 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
4741 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
4801 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
4861 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
4921 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
4981 cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
5041 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
5101 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
5161 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
5221 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
5281 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
5341 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
5401 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
5461 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
5521 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
5581 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
5641 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
5701 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
5761 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
5821 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
5881 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
5941 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
6001 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
6061 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct
6121 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
6181 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
6241 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
6301 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
6361 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
6421 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
6481 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
6541 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
6601 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
6661 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
6721 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
6781 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
6841 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
6901 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
6961 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
7021 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
7081 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
7141 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
7201 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
7261 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
7321 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
7381 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
7441 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
7501 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
7561 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
7621 aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa
7681 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
7741 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
7801 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
7861 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
7921 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
7981 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
8041 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
8101 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
8161 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
8221 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
8281 tccacatcat aggttgtccc tttataccgg ctgtccgtca tttttaaata taggttttca
8341 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
8401 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
8461 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
8521 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
8581 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
8641 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
8701 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
8761 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
8821 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
8881 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
8941 gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga
9001 ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata caaatacata
9061 ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt
9121 atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccg
9181 gtcggcatct actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat
9241 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg
9301 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag
9361 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
9421 gcatatacgc ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
9481 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt
9541 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt
9601 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt
9661 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
9721 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc
9781 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
9841 gatcggccgc agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt
9901 cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca
9961 ggctctcgct aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
10021 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga
10081 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct
10141 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg
10201 tgagttcagg ctttttcata tctcattgcc ccccggatct gcgaaagctc gagagagata
10261 gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt
10321 atatagagga aggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
10381 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
10441 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
10501 agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct
10561 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
10621 ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga
10681 gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgaagacgt
10741 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
10801 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
10861 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
10921 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
10981 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc
11041 aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
11101 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
11161 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
11221 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattac
