启动子： CaMV 35S promoter
终止子： NOS terminator
质粒大小： 10832bp
质粒标签： N-HA
原核抗性： 卡那霉素Kan
筛选标记： 潮霉素Hyg
克隆菌株： 大肠杆菌DB3.1
培养条件： 37℃，有氧，LB
pCXSN-HA质粒是一个植物过表达质粒，35S启动子驱动HA和目的基因融合表达，在ccdb致死基因的作用下，克隆基因变得更加容易，阳性率高。
序列：
ORIGIN
1 gaattccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
241 tgccaaatgt ttgaacgatc ggggaaattc gctagtggat ccccaatact tgtatggggc
301 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
361 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
421 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
481 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
541 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
601 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
661 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
721 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
781 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
841 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
901 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
961 ctgcaggtcg acccatacaa gtattggagc gtaatctgga acatcgtatg ggtacatgga
1021 tccccctcga gtatcgttcg taaatggtga aaattttcag aaaattgctt ttgctttaaa
1081 agaaatgatt taaattgctg caatagaagt agaatgcttg attgcttgag attcgtttgt
1141 tttgtatatg ttgtgttgag gtcgaggtcc tctccaaatg aaatgaactt ccttatatag
1201 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
1261 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
1321 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct
1381 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa
1441 taaagtgaca gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt
1501 gaaaagtctc aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga
1561 caagtgtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg
1621 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
1681 cggcagaggc atcttcaacg atggcctttc ctttatcgca atgatggcat ttgtaggagc
1741 caccttcctt ttccactatc ttcacaataa agtgacagat agctgggcaa tggaatccga
1801 ggaggtttcc ggatattacc ctttgttgaa aagtctcaat tgccctttgg tcttctgaga
1861 ctgtatcttt gatatttttg gagtagacaa gtgtgtcgtg ctccaccatg ttgacctgca
1921 ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
1981 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
2041 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca
2101 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
2161 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa
2221 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
2281 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
2341 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
2401 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
2461 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
2521 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
2581 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
2641 cccggagctg gccaggatgc ttgaccacct agccctggcg acgttgtgac agtgaccagg
2701 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
2761 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
2821 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
2881 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
2941 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
3001 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
3061 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
3121 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
3181 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
3241 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
3301 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
3361 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
3421 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
3481 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
3541 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
3601 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
3661 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
3721 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
3781 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
3841 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
3901 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
3961 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
4021 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
4081 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
4141 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
4201 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
4261 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
4321 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
4381 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
4441 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg
4501 caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc
4561 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg
4621 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
4681 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga
4741 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
4801 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
4861 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
4921 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
4981 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
5041 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
5101 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
5161 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
5221 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
5281 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
5341 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
5401 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
5461 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
5521 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
5581 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
5641 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc
5701 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
5761 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
5821 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
5881 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc
5941 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc
6001 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac
6061 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
6121 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
6181 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
6241 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
6301 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
6361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
6421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
6481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
6541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
6601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
6661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
6721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
6781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
6841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
6901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
6961 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
7021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
7081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
7141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
7201 tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
7261 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
7321 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
7381 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
7441 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
7501 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
7561 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
7621 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
7681 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
7741 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
7801 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
7861 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
7921 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
7981 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
8041 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
8101 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
8161 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
8221 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
8281 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
8341 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
8401 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
8461 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc
8521 gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt
8581 tattgataga agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca
8641 catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc acacattatt
8701 atggagaaac tcgagcttgt cgatcgacag atccggtcgg catctactct atttctttgc
8761 cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
8821 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
8881 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
8941 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gtcgtggcga
9001 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
9061 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
9121 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
9181 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
9241 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
9301 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
9361 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
9421 agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
9481 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctaaact ccccaatgtc
9541 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
9601 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
9661 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa
9721 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatatctca
9781 ttgccccccg gatctgcgaa agctcgagag agatagattt gtagagagag actggtgatt
9841 tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaaggtc ttgcgaagga
9901 tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt
9961 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
10021 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
10081 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
10141 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
10201 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
10261 atgttatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
10321 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg
10381 atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc
10441 cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
10501 ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg
10561 gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac
10621 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
10681 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
10741 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
10801 ttcacacagg aaacagctat gaccatgatt ac
