启动子： CaMV 35S promoter
终止子： NOS terminator
质粒大小： 10829bp
质粒标签： N-FLAG
原核抗性： 卡那霉素Kan
筛选标记： 潮霉素Hyg
克隆菌株： 大肠杆菌DB3.1
培养条件： 37℃，有氧，LB
pCXSN-FLAG质粒是一个植物过表达质粒，35S启动子驱动FLAG标签和目的基因融合表达，在ccdb致死基因的作用下，克隆基因变得更加容易，阳性率高。
序列：
ORIGIN
1 gaattccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
241 tgccaaatgt ttgaacgatc ggggaaattc gctagtggat ccccaatact tgtatggggc
301 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
361 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
421 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
481 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
541 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
601 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
661 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
721 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
781 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
841 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
901 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
961 ctgcaggtcg acccatacaa gtattggctt atcatcatca tccttgtaat ccatggatcc
1021 ccctcgagta tcgttcgtaa atggtgaaaa ttttcagaaa attgcttttg ctttaaaaga
1081 aatgatttaa attgctgcaa tagaagtaga atgcttgatt gcttgagatt cgtttgtttt
1141 gtatatgttg tgttgaggtc gaggtcctct ccaaatgaaa tgaacttcct tatatagagg
1201 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
1261 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
1321 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttcaacg atggcctttc
1381 ctttatcgca atgatggcat ttgtaggagc caccttcctt ttccactatc ttcacaataa
1441 agtgacagat agctgggcaa tggaatccga ggaggtttcc ggatattacc ctttgttgaa
1501 aagtctcaat tgccctttgg tcttctgaga ctgtatcttt gatatttttg gagtagacaa
1561 gtgtgtcgtg ctccaccatg ttatcacatc aatccacttg ctttgaagac gtggttggaa
1621 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
1681 cagaggcatc ttcaacgatg gcctttcctt tatcgcaatg atggcatttg taggagccac
1741 cttccttttc cactatcttc acaataaagt gacagatagc tgggcaatgg aatccgagga
1801 ggtttccgga tattaccctt tgttgaaaag tctcaattgc cctttggtct tctgagactg
1861 tatctttgat atttttggag tagacaagtg tgtcgtgctc caccatgttg acctgcaggc
1921 atgcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
1981 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
2041 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc tagagcagct
2101 tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga
2161 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataacgga tatttaaaag
2221 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
2281 tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
2341 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
2401 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
2461 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
2521 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
2581 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
2641 ggagctggcc aggatgcttg accacctagc cctggcgacg ttgtgacagt gaccaggcta
2701 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
2761 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
2821 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
2881 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc
2941 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
3001 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
3061 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
3121 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
3181 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
3241 tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa
3301 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
3361 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
3421 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
3481 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
3541 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
3601 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
3661 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
3721 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
3781 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
3841 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
3901 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
3961 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
4021 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
4081 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
4141 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
4201 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
4261 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
4321 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
4381 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
4441 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa
4501 tggcactgga acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg
4561 tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg
4621 cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg
4681 atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc
4741 cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc
4801 gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag
4861 ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg
4921 ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg
4981 aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac
5041 acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc
5101 tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg
5161 ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga
5221 tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt
5281 accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt
5341 tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg
5401 cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca
5461 agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt
5521 tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg
5581 agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag
5641 caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa
5701 agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc
5761 ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact
5821 ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc
5881 gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg
5941 ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag
6001 gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc
6061 aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
6121 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
6181 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
6241 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
6301 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
6361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
6421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
6481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
6541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
6601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
6661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
6721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
6781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
6841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
6901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
6961 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
7021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
7081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
7141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
7201 attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
7261 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
7321 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
7381 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
7441 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
7501 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
7561 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
7621 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
7681 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
7741 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
7801 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
7861 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
7921 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
7981 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
8041 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
8101 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
8161 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
8221 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
8281 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
8341 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
8401 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
8461 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa
8521 ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat
8581 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
8641 gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca cattattatg
8701 gagaaactcg agcttgtcga tcgacagatc cggtcggcat ctactctatt tctttgccct
8761 cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt
8821 ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg
8881 gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa
8941 gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagtc gtggcgatcc
9001 tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca
9061 cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct
9121 ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc
9181 cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag
9241 agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg
9301 gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg
9361 tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatagc
9421 ctccgcgacc ggttgtagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac
9481 accctgtgca cggcgggaga tgcaataggt caggctctcg ctaaactccc caatgtcaag
9541 cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta
9601 gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct
9661 gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt
9721 ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca tatctcattg
9781 ccccccggat ctgcgaaagc tcgagagaga tagatttgta gagagagact ggtgatttca
9841 gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaaggtcttg cgaaggatag
9901 tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa
9961 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
10021 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
10081 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
10141 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
10201 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
10261 ttatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
10321 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
10381 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
10441 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
10501 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
10561 tagacgagag tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc
10621 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
10681 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
10741 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
10801 acacaggaaa cagctatgac catgattac
