启动子： Ubi promoter
终止子： NOS terminator
质粒大小： 12041bp
质粒标签： N-Myc
原核抗性： 卡那霉素Kan
筛选标记： 潮霉素Hyg
克隆菌株： 大肠杆菌DB3.1
培养条件： 37℃，有氧，LB
pCXUN-Myc质粒是一个植物过表达质粒，UBi启动子驱动Myc标签和目的基因融合表达。空载体用XcmI酶切后，会变成T载体，可连入带A的PCR产物，其携带的ccdb致死基因可显著提高基因克隆的阳性率。
序列：
ORIGIN
1 gaattcgagc tcggtacccc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
61 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcccgatcta
121 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
181 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
241 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
301 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
361 aacgatcggg gaaattcgga tccccaatac ttgtatgggg cttactaaaa gccagataac
421 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
481 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
541 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
601 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
661 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
721 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
781 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
841 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
901 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
961 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
1021 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gacccataca
1081 agtattggaa gatcttcttc agaaatcaac ttttgttcca tggatccccc gggctgcaga
1141 agtaacacca aacaacaggg tgagcatcga caaaagaaac agtaccaagc aaataaatag
1201 cgtatgaagg cagggctaaa aaaatccaca tatagctgct gcatatgcca tcatccaagt
1261 atatcaagat caaaataatt ataaaacata cttgtttatt ataatagata ggtactcaag
1321 gttagagcat atgaatagat gctgcatatg ccatcatgta tatgcatcag taaaacccac
1381 atcaacatgt atacctatcc tagatcgata tttccatcca tcttaaactc gtaactatga
1441 agatgtatga cacacacata cagttccaaa attaataaat acaccaggta gtttgaaaca
1501 gtattctact ccgatctaga acgaatgaac gaccgcccaa ccacaccaca tcatcacaac
1561 caagcgaaca aaaagcatct ctgtatatgc atcagtaaaa cccgcatcaa catgtatacc
1621 tatcctagat cgatatttcc atccatcatc ttcaattcgt aactatgaat atgtatggca
1681 cacacataca gatccaaaat taataaatcc accaggtagt ttgaaacaga attctactcc
1741 gatctagaac gaccgcccaa ccagaccaca tcatcacaac caagacaaaa aaaagcatga
1801 aaagatgacc cgacaaacaa gtgcacggca tatattgaaa taaaggaaaa gggcaaacca
1861 aaccctatgc aacgaaacaa aaaaaatcat gaaatcgatc ccgtctgcgg aacggctaga
1921 gccatcccag gattccccaa agagaaacac tggcaagtta gcaatcagaa cgtgtctgac
1981 gtacaggtcg catccgtgta cgaacgctag cagcacggat ctaacacaaa cacggatcta
2041 acacaaacat gaacagaagt agaactaccg ggccctaacc atggaccgga acgccgatct
2101 agagaaggta gagagggggg gggggggagg acgagcggcg taccttgaag cggaggtgcc
2161 gacgggtgga tttgggggag atctggttgt gtgtgtgtgc gctccgaaca acacgaggtt
2221 ggggaaagag ggtgtggagg gggtgtctat ttattacggc gggcgaggaa gggaaagcga
2281 aggagcggtg ggaaaggaat cccccgtagc tgccgtgccg tgagaggagg aggaggccgc
2341 ctgccgtgcc ggctcacgtc tgccgctccg ccacgcattt ctggatgccg acagcggagc
2401 aagtccaacg gtggagcgga actctcgaga ggggtccaga ggcagcgaca gagatgccgt
2461 gccgtctgct tcgcttggcc cgacgcgacg ctgctggttc gctggttggt gtccgttaga
2521 ctcgtcgacg gcgtttaaca ggctggcatt atctactcga aacaagaaaa atgtttcctt
2581 agttttttta atttcttaaa gggtatttgt ttaattttta gtcactttat tttattctat
2641 tttatatcta aattattaaa taaaaaaact aaaatagagt tttagttttc ttaatttaga
2701 ggctaaaata gaataaaata gatgtactaa aaaaattagt ctataaaaac cattaaccct
2761 aaaccctaaa tggatgtact aataaaatgg atgaagtatt atataggtga agctatttgc
2821 aaaaaaaaag gagaacacat gcacactaaa aagataaaac tgtagagtcc tgttgtcaaa
2881 atactcaatt gtcctttaga ccatgtctaa ctgttcattt atatgattct ctaaaacact
2941 gatattattg tagtactata gattatatta ttcgtagagt aaagtttaaa tatatgtata
3001 aagatagata aactgcactt caaacaagtg tgacaaaaaa aatatgtggt aattttttat
3061 aacttagaca tgcaatgctc attatctcta gagaggggca cgaccgggtc acgctgcact
3121 gcaggaattc gatatcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
3181 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
3241 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
3301 gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag
3361 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg
3421 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
3481 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
3541 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
3601 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
3661 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
3721 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
3781 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
3841 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta gccctggcga cgttgtgaca
3901 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
3961 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
4021 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
4081 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc
4141 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa
4201 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc
4261 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt
4321 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa
4381 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg
4441 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc
4501 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct
4561 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
4621 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
4681 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
4741 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
4801 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
4861 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
4921 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
4981 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcaa ccgccgacct
5041 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
5101 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
5161 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
5221 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
5281 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
5341 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
5401 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
5461 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
5521 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
5581 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
5641 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg
5701 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac
5761 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
5821 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
5881 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
5941 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
6001 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
6061 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
6121 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
6181 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
6241 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
6301 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
6361 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
6421 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
6481 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
6541 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
6601 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
6661 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
6721 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
6781 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
6841 aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca
6901 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
6961 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
7021 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
7081 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
7141 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
7201 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
7261 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
7321 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
7381 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
7441 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
7501 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
7561 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
7621 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
7681 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
7741 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
7801 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
7861 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
7921 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
7981 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
8041 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
8101 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
8161 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
8221 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
8281 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
8341 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
8401 ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa tattttattt
8461 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
8521 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
8581 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
8641 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
8701 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
8761 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
8821 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
8881 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
8941 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
9001 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggttg
9061 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
9121 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
9181 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
9241 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
9301 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
9361 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac
9421 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
9481 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
9541 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
9601 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
9661 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga
9721 attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat
9781 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
9841 tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca
9901 cacattatta tggagaaact cgagcttgtc gatcgacaga tccggtcggc atctactcta
9961 tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
10021 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
10081 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
10141 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
10201 tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
10261 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
10321 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
10381 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
10441 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
10501 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
10561 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
10621 cgcatccata gcctccgcga ccggttgtag aacagcgggc agttcggttt caggcaggtc
10681 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctaaactc
10741 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata
10801 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc
10861 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac
10921 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt
10981 catatctcat tgccccccgg atctgcgaaa gctcgagaga gatagatttg tagagagaga
11041 ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt ccttatatag aggaaggtct
11101 tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc
11161 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
11221 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
11281 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
11341 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
11401 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
11461 tgctccacca tgttatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
11521 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
11581 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
11641 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
11701 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
11761 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa
11821 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
11881 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
11941 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
12001 gataacaatt tcacacagga aacagctatg accatgatta c
