pGM1190链霉菌表达质粒

  • 货号:KL-ZL1615-2ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL1615-2ug pGM1190链霉菌表达质粒 2ug

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货号 名称 规格
KL-ZL1615 pGM1190链霉菌表达质粒

2ug

启动子: TipA promoter 质粒大小: 7089bp 质粒标签: N-6xHis/thrombin site/T7 tag,C-6xHis 原核抗性: 安普霉素Apr 克隆菌株: 大肠杆菌DH5α 培养条件: 有氧,LB,37℃ 表达宿主: 链霉菌
pGM1190是一个链霉菌表达载体,通过TipA 启动子驱动目的基因的表达。转化进链霉素的时候请用35℃的温度培养。OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces,replicates also in Streptomyces (below 35°C).

序列:
ORIGIN
1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
121 cgaagcggta cgcgaacccg tgccgggctg cggcggatcg cggcagcagg agctccccgc
181 ccgccacggc tggctggatg gtcgcctcac gcgcccgcag gggtgacggc gtgggtgcgg
241 cggtcagccc cgtgatcggg cgtcagacgg ccgctgaggg accgcgacca cccctccctt
301 cccgcctgcc gtctttcacc gcccggccgg tccgcagacg ccgtacggcc aggccacgga
361 ggattacggg cagatggcgc ccacccggca cccgggctgg ggcaccctgg acagcacgaa
421 ggcccgcccc actcaccggg gcgggccttc gcgttgagct agagttcccg ccagcctcgc
481 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
541 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
601 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
661 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa tgcagctcac
721 ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa
781 aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt
841 atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca
901 gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt
961 ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc
1021 cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctgc gctgggtccg
1081 ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc
1141 acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat
1201 gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc
1261 atctctgatc cattgcccct gccacctcac tcgcctgcaa gcccggtcgc ccgtgtccat
1321 gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat
1381 cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag
1441 gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc
1501 ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc
1561 tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga
1621 gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc
1681 cagtcgattg gctgagctca taagttccta ttccgaagtt cctattcgaa atgaccgacc
1741 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
1801 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
1861 tgctggagtt cttcgcccac cccaaaagga tctaggtgaa gatccttttt gataatctca
1921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
1981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
2041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
2101 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
2161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
2221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
2281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
2341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
2401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
2461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
2521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
2581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
2641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
2701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
2761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
2821 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
2881 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
2941 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattgt
3001 acgtaggcgt cagctacgat gttccgggga ctgctgatcc ggtcagcagg tggaagaggg
3061 actggattcc aaagttctca atgctgcttg ctgttcttga atggggggtc gttgacgacg
3121 acatggctcg attggcgcga caagttgctg cgattctcac caataaaaaa cgcccggcgg
3181 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg accggatcgg
3241 ggatctgggc tgagggagcc gacggcacgc ggcggctcac ggcgtggcac gcggaacgtc
3301 cgggcttgca cctcacgtca cgtgaggagg cagcgtggac ggcgtcagag aagggagcgg
3361 accatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc
3421 catatggcta gcatgactgg tggacagcaa atgggtcgcg gatccgaatt cgagctccgt
3481 cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
3541 cccagatcta aagttttgtc gtctttccag acgttagtaa atgaattttc tgtatgaggt
3601 tttgctaaac aactttcaac agtttcagcg gagtgagaat agaaaggaac aactaaagga
3661 attgcgaata ataatttttt cacgttgaaa atctccaaaa aaaaaggctc caaaaggagc
3721 ctttaattgt atcggtttat cagcttgctt tcgaggtgaa tttcttaaac agcttgatac
3781 cgatagttgc gccgacaatg acaacaacca tcgcccacgc ataaccgata tattcggtcg
3841 ctgaggcttg cagggagtca aaggccgctt ttgcgggatc tcgtcgaagg cactagaggc
3901 ctatatgcgg ggatcgaccg cgcgggtccc ggacggggaa gagcggggag ctttgccaga
3961 gagcgacgac ttccccttgc gttggtgatt gccggtcagg gcagccatcc gccatcgtcg
4021 cgtagggtgt cacaccccag gaatcgcgtc actgaacaca gcagccggta ggacgaccat
4081 gactgagttg gacaccatcg caaatccgtc cgatcccgcg gtgcagcgga tcatcgatgt
4141 caccaagccg tcgcgatcca acataaagac aacgttgatc gaggacgtcg agcccctcat
4201 gcacagcatc gcggccgggg tggagttcat cgaggtctac ggcagcgaca gcagtccttt
4261 tccatctgag ttgctggatc tgtgcgggcg gcagaacata ccggtccgcc tcatcgactc
4321 ctcgatcgtc aaccagttgt tcaaggggga gcggaaggcc aagacattcg gcatcgcccg
4381 cgtccctcgc ccggccaggt tcggcgatat cgcgagccgg cgtggggacg tcgtcgttct
4441 cgacggggtg aagatcgtcg ggaacatcgg cgcgatagta cgcacgtcgc tcgcgctcgg
4501 agcgtcgggg atcatcctgg tcgacagtga catcaccagc atcgcggacc ggcgtctcca
4561 aagggccagc cgaggttacg tcttctccct tcccgtcgtt ctctccggtc gcgaggaggc
4621 catcgccttc attcgggaca gcggtatgca gctgatgacg ctcaaggcgg atggcgacat
4681 ttccgtgaag gaactcgggg acaatccgga tcggctggcc ttgctgttcg gcagcgaaaa
4741 gggtgggcct tccgacctgt tcgaggaggc gtcttccgcc tcggtttcca tccccatgat
4801 gagccagacc gagtctctca acgtttccgt ttccctcgga atcgcgctgc acgagaggat
4861 cgacaggaat ctcgcggcca accgataagc gcctctgttc ctcggacgct cggttgctcg
4921 acctcgattc gtcagtgatg atcaacagga cccagaaatg gcacgagccc gggattggcc
4981 tcccgggctc gccgcccccg aaccggcgca gcatgccgag tgaggacgtt tgctctgtga
5041 cgagcgtacg agagaacagc gcgtcgacac caccgcgccc cgccggaccg aagtgccact
5101 gcggcacccc gatcgtccac atccccggga agcgccccaa aatctactgc tcggacgcct
5161 gccgaaagcg cgcaaagcgt ctcattgcgg accagtcacg cagcgtagca agcacgacta
5221 cagcgggtaa tccggacgcg ggctttagta gtaagggaaa atctccgtcc actgcaggtc
5281 agagcgctgt aactggaacg ggttctaccc caaacgaggg tgccgcgaca gccgggcggg
5341 aggacccgcg cgacgagcgg ttcgcccggc gggaccggca ccagacggtg tcgctgaacg
5401 aggcgttcaa gggctgcggg acccggctca ctgccgggac ggccgagctg atgtggaagc
5461 cgggcgaggc gacgtggggc aacacgtgcc gctgcaacaa cgtccacacc tgcccgtggt
5521 gcatgagccg catcctggcc gtacggggct cgaacgtgca gctcgccgcc gacggtctcg
5581 cggacgccgg gtacgggctg caccttggca cgaacacgtt gcgccacttc gagcgcatgg
5641 cgttcggcac cgtccgcaag ggaatgcggc acggcctggt cgcggtcttg cacgacggct
5701 ggaagggcgc gtacggctcg tccggccgcc gctggcgcac gatgcgcgac gacttcggga
5761 tcatcggcta cgagcgggcc ttcgaggaca ccttcggctg gggctccggc tggcacctgc
5821 actggcacac gctctgggtc acccgcgagg tgctcggccc ggacgcccag gccgcgttcc
5881 gcgacgcact cgccggagcc tgggccgccg gggtcgaatc cgcgggcggc tacaccgtca
5941 gcgagacgtg cgaccggccc ggttgctcct gtgagggcaa gggccacggc accgacgtgc
6001 gcccactcaa cggcgcggac gccgctgacg gcgatgcggg caagcaggcc cgctacctct
6061 acaaggacgg cgacaagacc aagggcggcg tcgccaagat cggcctggag ctggccggac
6121 agaacttcaa ggccggtcgc ggcgacgacc gcatgggacc gctcgacctg ggcgacgcgg
6181 cggccgccga gctgcagcgg cttcgtcggc ccggcccgtt cgtcgagaag taccgcgagc
6241 gcgagttcgg cgtcttccag gtccgcaagc actaccggtc gcagaacctg aaccggctga
6301 tcaaggaact cggcatccag caggacgtcc gcaccgagga ggagatcacc gacgacaccg
6361 agggcctggt cgccatcgcg gtcatcccgg cctacatctg gtaccgatac atcgcccgcg
6421 tcgccggacg tcgcctcgac ctcatcaagg tcgccgagac gtacggccta ccgggcgtcc
6481 ggcggctcgt cgagtcctgg ggcctggtgt ggggcaagga cgtcctggac ccgcccgccc
6541 cggaagcgcc ggccgcgccg ggcgaccttg acgctgacca gatgaggttc gaggtcatgt
6601 ccgaggagga agctgctttc cgcgaggcgc gccggaaggc caacgaggcc cgcacggagg
6661 agctggccgc ttcgctcgac cgagtgcgtc agccgaagaa ggaggcgatc cgcccgacga
6721 tcagccttcg gaagcgcctg aagccgaagc cggtcaccgt cgacgtcaag actcccccgc
6781 ccggcgccgc gtcgcccgtg tgccgccgct gcaagggcaa gctcgccccg gtactccagc
6841 cctggggccg gctccccgga gactgcctcc gcgtcgacac agcggtcgcg tagggccgct
6901 gagggaccgc catgagaccg ccaccccgga catccgggtg gcggtctcat ggcggtcccg
6961 gcgcgaaagc cgagggtctg cctgccgtga ggtggccggc ggacctgggt tactgctgga
7021 atcccgcgcc cagtccgagc gtccgccggc gcttttcacc tggctccggg tgctcgtcga
7081 tgagcacca

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