pCAMBIA1391植物表达质粒

复制子： pVS1 oriV，pUC ori 终止子： NOS terminator 质粒大小： 10658bp 原核抗性： 卡那霉素Kan 筛选标记： 潮霉素Hyg 克隆菌株： 大肠杆菌Stbl3 培养条件： 37℃，有氧，LB

pCAMBIA1391是一个植物启动子检测报告载体，可通过MCS克隆进需要研究的启动子。 The pCambia vector backbone is derived from the pPZP vectors. The pCambia1391 vector offers：  High copy number in E.coli for high DNA yields  pVS1 replicon for high stability in Agrobacterium  Small size  Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest  Bacterial selection with kanamycin  Plant selection with hygromycin B  Simple means to construct translational fusions to gusA reporter genes Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins. Designed to utilize gusA as a true reporter of gene expression by fusion construction this vector contains a promoterless, non-intron gusA gene that retains the initiation codon of the NcoI site. This permits simple construction of carboxy-terminus protein fusions to gusA.

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