pECMV-CCNL2-3×Flag人源基因质粒

  • 货号:KL-ZL1609-2ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL1609-2ug pECMV-CCNL2-3×Flag人源基因质粒 2ug

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货号 名称 规格
KL-ZL1609 pECMV-CCNL2-3×Flag人源基因质粒

2ug

别名: NM_030937.5 启动子: CMV promoter 终止子: bGH poly(A) signal 质粒大小: 7043bp 质粒标签: C-3×FLAG 原核抗性: 氨苄青霉素Amp 筛选标记: 新霉素Neo/G418 克隆菌株: 大肠杆菌DH5α 培养条件: 37℃,有氧,LB 备注: NM_030937.5
pECMV-CCNL2-3×FLAG质粒是一个哺乳细胞过表达质粒,CMV启动子驱动CCNL2人源基因和3×FLAG标签融合表达。

序列:
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggcggcgg cggcggcggc
961 ggctggtgct gcagggtcgg cagctcccgc ggcagcggcc ggcgccccgg gatctggggg
1021 cgcaccctca gggtcgcagg gggtgctgat cggggacagg ctgtactccg gggtgctcat
1081 caccttggag aactgcctcc tgcctgacga caagctccgt ttcacgccgt ccatgtcgag
1141 cggcctcgac accgacacag agaccgacct ccgcgtggtg ggctgcgagc tcatccaggc
1201 ggccggtatc ctgctccgcc tgccgcaggt ggccatggct accgggcagg tgttgttcca
1261 gcggttcttt tataccaagt ccttcgtgaa gcactccatg gagcatgtgt caatggcctg
1321 tgtccacctg gcttccaaga tagaagaggc cccaagacgc atacgggacg tcatcaatgt
1381 gtttcaccgc cttcgacagc tgagagacaa aaagaagccc gtgcctctac tactggatca
1441 agattatgtt aatttaaaga accaaattat aaaggcggaa agacgagttc tcaaagagtt
1501 gggtttctgc gtccatgtga agcatcctca taagataatc gttatgtacc ttcaggtgtt
1561 agagtgtgag cgtaaccaac acctggtcca gacctcatgg aattacatga acgacagcct
1621 tcgcaccgac gtcttcgtgc ggttccagcc agagagcatc gcctgtgcct gcatttatct
1681 tgctgcccgg acgctggaga tccctttgcc caatcgtccc cattggtttc ttttgtttgg
1741 agcaactgaa gaagaaattc aggaaatctg cttaaagatc ttgcagcttt atgctcggaa
1801 aaaggttgat ctcacacacc tggagggtga agtggaaaaa agaaagcacg ctatcgaaga
1861 ggcaaaggcc caagcccggg gcctgttgcc tgggggcaca caggtgctgg atggtacctc
1921 ggggttctct cctgccccca agctggtgga atcccccaaa gaaggtaaag ggagcaagcc
1981 ttccccactg tctgtgaaga acaccaagag gaggctggag ggcgccaaga aagccaaggc
2041 ggacagcccc gtgaacggct tgccaaaggg gcgagagagt cggagtcgga gccggagccg
2101 tgagcagagc tactcgaggt ccccatcccg atcagcgtct cctaagagga ggaaaagtga
2161 cagcggctcc acatctggtg ggtccaagtc gcagagccgc tcccggagca ggagtgactc
2221 cccaccgaga caggcccccc gcagcgctcc ctacaaaggc tctgagattc ggggctcccg
2281 gaagtccaag gactgcaagt acccccagaa gccacacaag tctcggagcc ggagttcttc
2341 ccgttctcga agcaggtcac gggagcgggc ggataatccg ggaaaataca agaagaaaag
2401 tcattactac agagatcagc gacgagagcg ctcgaggtcg tatgaacgca caggccgtcg
2461 ctatgagcgg gaccaccctg ggcacagcag gcatcggagg gaattctgca gatatccagc
2521 acagtggcgg ccgctcgagg actacaaaga ccatgacggt gattataaag atcatgacat
2581 cgactacaag gatgacgatg acaagtagtg agggcccgtt taaacccgct gatcagcctc
2641 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
2701 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
2761 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
2821 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
2881 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
2941 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
3001 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
3061 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
3121 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
3181 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
3241 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
3301 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
3361 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
3421 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
3481 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
3541 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
3601 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
3661 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
3721 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
3781 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
3841 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
3901 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
3961 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
4021 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
4081 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
4141 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
4201 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
4261 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
4321 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
4381 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
4441 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
4501 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
4561 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
4621 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
4681 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
4741 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
4801 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
4861 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
4921 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
4981 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
5041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
5101 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
5161 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
5221 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
5281 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
5341 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
5401 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
5461 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
5521 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
5581 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
5641 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
5701 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
5761 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
5821 gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
5881 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
5941 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
6001 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
6061 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
6121 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
6181 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
6241 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
6301 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
6361 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
6421 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
6481 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
6541 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
6601 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
6661 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
6721 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
6781 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
6841 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
6901 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
6961 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
7021 cccgaaaagt gccacctgac gtc

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