pLVCT-tTR-KRAB慢病毒干扰质粒,其他信息可咨询销售人员：QQ：1722105945 电话：021-57880797 13248085519 （微信同号）
Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation.
序列：
polyA_signal 12580..12714
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
61 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
121 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
181 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
241 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
301 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
361 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
421 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
661 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
721 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
781 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
841 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
901 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
961 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
1021 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
1081 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
1141 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc
2041 ctcgaccatc gatgtcgatc gacattgatt attgactagt tattaatagt aatcaattac
2101 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
2161 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
2221 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
2281 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
2341 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
2401 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
2461 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
2521 ttaattattt tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg
2581 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
2641 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
2701 gaagcgcgcg gcgggcggga gtcgctgcgt tgccttcgcc ccgtgccccg ctccgcgccg
2761 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
2821 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctc gtttcttttc
2881 tgtggctgcg tgaaagcctt aaagggctcc gggagggccc tttgtgcggg ggggagcggc
2941 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggccc gcgctgcccg
3001 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcgtg tgcgcgaggg
3061 gagcgcggcc gggggcggtg ccccgcggtg cgggggggct gcgaggggaa caaaggctgc
3121 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa
3181 cccccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
3241 ccgtgcgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
3301 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc
3361 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
3421 tgcgagaggg cgcagggact tcctttgtcc caaatctggc ggagccgaaa tctgggaggc
3481 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
3541 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
3601 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
3661 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
3721 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
3781 taaatttaat taatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
3841 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa tttaaagaat
3901 tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgaggttt
3961 aaactacggg atccaggcct aagcttacgc gtcctagcgc taccggtcgc caccatggtg
4021 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
4081 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
4141 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
4201 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
4261 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
4321 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
4381 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
4441 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
4501 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
4561 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
4621 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
4681 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc
4741 agatctcgac tagctagtag ctagctagct agtcgagctc aagcttcgaa ttcccgggct
4801 gcaggaattc cgcccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
4861 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
4921 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
4981 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
5041 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
5101 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
5161 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
5221 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
5281 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac
5341 ggggacgtgg ttttcctttg aaaaacacga tgataatacc atggctagat tagataaaag
5401 taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac
5461 ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa
5521 taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt
5581 ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag
5641 atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga
5701 aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact
5761 agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga
5821 agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc
5881 gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt
5941 attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc
6001 gccaaaaaag aagagaaagg tcgacggcgg tggtgctttg tctcctcagc actctgctgt
6061 cactcaagga agtatcatca agaacaagga gggcatggat gctaagtcac taactgcctg
6121 gtcccggaca ctggtgacct tcaaggatgt atttgtggac ttcaccaggg aggagtggaa
6181 gctgctggac actgctcagc agatcgtgta cagaaatgtg atgctggaga actataagaa
6241 cctggtttcc ttgggttatc agcttactaa gccagatgtg atcctccggt tggagaaggg
6301 agaagagccc tggctggtgg agagagaaat tcaccaagag acccatcctg attcagagac
6361 tgcatttgaa atcaaatcat cagtttaagg atctcgacta gctagtagct agctagctag
6421 tcgagctcaa cttcgaattc gatgggacta gtcatatgat aatcaacctc tggattacaa
6481 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
6541 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
6601 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
6661 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
6721 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
6781 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
6841 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
6901 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
6961 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
7021 tcggatctcc ctttgggccg cctccccgca tcggtacgta tggccaggta cctttaagac
7081 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
7141 aagggctaat tcactcccaa cgaagacaag atctcgagtt taccactccc tatcagtgat
7201 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
7261 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
7321 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
7381 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
7441 cctatcagtg atagagaaaa gtgaaagtcg gatcgatctg ctttttgctt gtactgggtc
7501 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
7561 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
7621 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
7681 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
7741 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
7801 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
7861 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
7921 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
7981 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
8041 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
8101 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
8161 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
8221 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
8281 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
8341 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
8401 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
8461 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
8521 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
8581 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
8641 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
8701 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
8761 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
8821 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
8881 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
8941 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
9001 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
9061 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
9121 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
9181 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
9241 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
9301 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
9361 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
9421 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
9481 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
9541 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
9601 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
9661 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
9721 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
9781 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
9841 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
9901 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
9961 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
10021 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
10081 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
10141 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
10201 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
10261 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
10321 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
10381 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
10441 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
10501 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
10561 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
10621 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
10681 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
10741 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
10801 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
10861 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
10921 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
10981 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
11041 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
11101 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
11161 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
11221 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
11281 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
11341 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
11401 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
11461 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
11521 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
11581 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
11641 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
11701 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
11761 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
11821 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
11881 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
11941 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
12001 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
12061 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
12121 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
12181 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
12241 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
12301 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
12361 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
12421 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
12481 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
12541 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
12601 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
12661 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
12721 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
12781 aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
12841 aagaaattgt atttgttaaa tatgtactac aaactta
