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pT7-His-CL2人源基因质粒

  • 货号：KL-ZL1561-5ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL1561-5ug pT7-His-CL2人源基因质粒 5ug

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》产品信息

货号 名称 规格
KL-ZL1561 pT7-His-CL2人源基因质粒

5ug

》产品描述

别名： NM_002982.3;CIRL2, ADGRL2, LEC1, LPH 原核抗性： 卡那霉素Kan 克隆菌株： 大肠杆菌DH5α 培养条件： 37℃

》产品简介

pT7-His-CL2是一个人源基因大肠表达质粒。

》产品图谱及序列


序列：
CDS complement(4253..5335)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter complement(5336..5413)
/gene="lacI"
/note="lacI promoter"
ORIGIN
1 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
61 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
121 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
181 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
241 atcatcatca tcatcacagc agcggccgcg gatccatgaa agtctctgcc gcccttctgt
301 gcctgctgct catagcagcc accttcattc cccaagggct cgctcagcca gatgcaatca
361 atgccccagt cacctgctgt tataacttca ccaataggaa gatctcagtg cagaggctcg
421 cgagctatag aagaatcacc agcagcaagt gtcccaaaga agctgtgatc ttcaagacca
481 ttgtggccaa ggagatctgt gctgacccca agcagaagtg ggttcaggat tccatggacc
541 acctggacaa gcaaacccaa actccgaaga cttaactcga gcaccaccac caccaccact
601 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
661 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
721 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
781 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
841 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
901 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
961 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
1021 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
1081 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
1141 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
1201 aatttcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
1261 aatacattca aatatgtatc cgctcatgaa ttaattctta gaaaaactca tcgagcatca
1321 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
1381 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
1441 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
1501 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
1561 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
1621 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
1681 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
1741 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
1801 ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
1861 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
1921 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
1981 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
2041 catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt
2101 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
2161 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
2221 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
2281 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
2341 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
2401 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
2461 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
2521 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
2581 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
2641 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
2701 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
2761 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
2821 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
2881 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
2941 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
3001 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
3061 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
3121 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
3181 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
3241 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta
3301 aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag
3361 ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag
3421 ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg
3481 ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca
3541 tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca
3601 gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca
3661 gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt
3721 ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt
3781 cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg
3841 ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat
3901 gcgcacccgt ggggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt
3961 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga
4021 caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc
4081 tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat
4141 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg
4201 tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc
4261 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
4321 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca
4381 acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg
4441 tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc
4501 tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact
4561 cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg
4621 gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt
4681 cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag
4741 ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt
4801 gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa
4861 tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg
4921 cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga
4981 cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta
5041 ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa
5101 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt
5161 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt
5221 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg
5281 tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca
5341 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc
5401 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag
5461 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
5521 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
5581 c

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