| 货号 | 产品名称 | 规格 | 库存 | 价格 | 数量 | 购买 |
|---|---|---|---|---|---|---|
| kl-zl-146-01 | pET-28c质粒 | NA |
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| pET-28c质粒 货号：kl-zl-146 |
| 启动子： T7/lac |
| 复制子： ColE1 ori，F1 ori |
| 终止子： T7 terminator |
| 质粒分类： 大肠杆菌载体；PET系列表达质粒 |
| 质粒大小： 5367bp |
| 质粒标签： ColE1 ori，F1 ori |
| 原核抗性： 卡那霉素Kan |
| 克隆菌株： DH5α |
| 培养条件： 37℃，有氧，LB |
| 表达宿主： BL21(DE3) |
| 诱导方式： IPTG或乳糖及其类似物 |
| 5'测序引物： T7：TAATACGACTCACTATAGGG |
| 3'测序引物： T7-ter：TGCTAGTTATTGCTCAGCGG |
| 质粒简介： |
| The pET-28a-c(+) vectors carry an N-terminal His•Tag®/ thrombin/ T7•Tag® configuration plus an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymeraseis shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer. |
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