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pBABE-Puro SV40 LT病毒基因哺乳表达质粒

货号：KL-ZL1285-01
培养条件：37℃，有氧，LB

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL1285-01	pBABE-Puro SV40 LT病毒基因哺乳表达质粒	NA		立即咨询	+ -

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pBABE-Puro SV40 LT病毒基因哺乳表达质粒货号：KL-ZL1285 规格：5ug
一,质粒描述：

质粒分类：基因文库质粒；其它基因质粒；其它种源质粒

质粒大小： 7273bp

原核抗性：氨苄青霉素Amp（100μg/ml）

筛选标记：嘌呤霉素Puro

克隆菌株： Stbl3等大肠杆菌

培养条件： 37℃，有氧，LB

表达宿主： 293T等哺乳细胞

诱导方式：无需诱导

备注：低拷贝
二,质粒简介：

序列：

promoter complement(7150..7254)

/gene="bla"

/label=AmpR promoter

ORIGIN

1 ccacctgcag cctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg

61 ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag

121 cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca

181 gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg

241 tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc

301 ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg

361 agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc

421 tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc

481 atttgggggc tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg

541 gaggcaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat

601 tttatgcgcc tgcgtcggta ctagttagct aactagctct gtatctggcg gacccgtggt

661 ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag ggactttggg

721 ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga

781 tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg

841 ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg

901 tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc

961 actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag

1021 tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt

1081 aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc

1141 aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg

1201 gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg

1261 cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct

1321 cgatcctccc tttatccagc cctcactcct tctctaggcg ccggccggat cccgcgaccg

1381 gtcgaccagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat

1441 ggaccttcta ggtcttgaaa ggagtgcctg ggggaatatt cctctgatga gaaaggcata

1501 tttaaaaaaa tgcaaggagt ttcatcctga taaaggagga gatgaagaaa aaatgaagaa

1561 aatgaatact ctgtacaaga aaatggaaga tggagtaaaa tatgctcatc aacctgactt

1621 tggaggcttc tgggatgcaa ctgagattcc aacctatgga actgatgaat gggagcagtg

1681 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga

1741 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga

1801 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag

1861 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa

1921 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat

1981 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa

2041 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag

2101 tgccttgact agagatccat tttctgttat tgaggaaagt ttgccaggtg ggttaaagga

2161 gcatgatttt aatccagaag aagcagagga aactaaacaa gtgtcctgga agcttgtaac

2221 agagtatgca atggaaacaa aatgtgatga tgtgttgtta ttgcttggga tgtacttgga

2281 atttcagtac agttttgaaa tgtgtttaaa atgtattaaa aaagaacagc ccagccacta

2341 taagtaccat gaaaagcatt atgcaaatgc tgctatattt gctgacagca aaaaccaaaa

2401 aaccatatgc caacaggctg ttgatactgt tttagctaaa aagcgggttg atagcctaca

2461 attaactaga gaacaaatgt taacaaacag atttaatgat cttttggata ggatggatat

2521 aatgtttggt tctacaggct ctgctgacat agaagaatgg atggctggag ttgcttggct

2581 acactgtttg ttgcccaaaa tggattcagt ggtgtatgac tttttaaaat gcatggtgta

2641 caacattcct aaaaaaagat actggctgtt taaaggacca attgatagtg gtaaaactac

2701 attagcagct gctttgcttg aattatgtgg ggggaaagct ttaaatgtta atttgccctt

2761 ggacaggctg aactttgagc taggagtagc tattgaccag tttttagtag tttttgagga

2821 tgtaaagggc actggagggg agtccagaga tttgccttca ggtcagggaa ttaataacct

2881 ggacaattta agggattatt tggatggcag tgttaaggta aacttagaaa agaaacacct

2941 aaataaaaga actcaaatat ttccccctgg aatagtcacc atgaatgagt acagtgtgcc

3001 taaaacactg caggccagat ttgtaaaaca aatagatttt aggcccaaag attatttaaa

3061 gcattgcctg gaacgcagtg agtttttgtt agaaaagaga ataattcaaa gtggcattgc

3121 tttgcttctt atgttaattt ggtacagacc tgtggctgag tttgctcaaa gtattcagag

3181 cagaattgtg gagtggaaag agagattgga caaagagttt agtttgtcag tgtatcaaaa

3241 aatgaagttt aatgtggcta tgggaattgg agttttagat tggctaagaa acagtgatga

3301 tgatgatgaa gacagccagg aaaatgctga taaaaatgaa gatggtgggg agaagaacat

3361 ggaagactca gggcatgaaa caggcattga ttcacagtcc caaggctcat ttcaggcccc

3421 tcagtcctca cagtctgttc atgatcataa tcagccatac cacatttgta gaggttttac

3481 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg

3541 ttgttgttaa cggggatccc agtgtggtgg tacgtaggaa ttcgccagca cagtggtcga

3601 ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa

3661 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc

3721 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc

3781 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct

3841 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga

3901 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttacc atgaccgagt

3961 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg

4021 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg

4081 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg

4141 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag

4201 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg

4261 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt

4321 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg

4381 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg

4441 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg

4501 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc

4561 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgata aaataaaaga

4621 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc

4681 tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt

4741 tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg

4801 taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa

4861 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc

4921 agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca

4981 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct

5041 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg

5101 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag

5161 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac

5221 ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta

5281 agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc

5341 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt

5401 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca

5461 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa

5521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat

5581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc

5641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc

5701 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt

5761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac

5821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg

5881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca

5941 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc

6001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa

6061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa

6121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac

6181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg

6241 cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt

6301 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact

6361 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat

6421 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg

6481 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg

6541 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat

6601 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc

6661 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt

6721 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc

6781 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga

6841 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc

6901 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa

6961 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta

7021 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg

7081 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg

7141 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat

7201 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt

7261 tccccgaaaa gtg

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