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pUC57-CNGA1-FLAG(NM_001142564.1)人源基因质粒

  • 货号:KL-ZL-1212-01
  • 培养条件:37℃,有氧,LB
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL-1212-01 pUC57-CNGA1-FLAG(NM_001142564.1)人源基因质粒 NA

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pUC57-CNGA1-FLAG(NM_001142564.1)人源基因质粒 货号:KL-ZL-1212 规格:5ug
一,质粒描述:

别名: NM_001142564.1,CNCG; CNCG1; CNG-1;
复制子: pUC ori
质粒分类: 基因文库质粒;人源基因质粒;人源克隆质粒
质粒大小: 4745bp
质粒标签: C-FLAG
原核抗性: 氨苄青霉素Amp
克隆菌株: 大肠杆菌DH5α
培养条件: 37℃,有氧,LB
5'测序引物: M13F:TGTAAAACGACGGCCAGT
3'测序引物: M13R:CAGGAAACAGCTATGACC
备注: GenBank: BC140399.1
二,质粒简介:
pUC57-CNGA1-FLAG质粒是克隆有CNGA1人源基因的非表达重组质粒,可用做扩增CNGA1基因的PCR模板,也可以用XhoI和EcoRI双酶切下CNGA1基因。
三,质粒图谱及序列:

序列:
promoter complement(4546..4650)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtctcga gatgaaacta tccatgaaga
421 acaatattat caatacacag cagtcttttg taaccatgcc caatgtgatt gtaccagata
481 ttgaaaagga aatacgaagg atggaaaatg gagcatgcag ctccttttct gaggatgatg
541 acagtgcctc tacatctgaa gaatcagaga atgaaaaccc tcatgcaagg ggttccttta
601 gttataagtc actcagaaag ggaggaccat cacagaggga gcagtacctg cctggtgcca
661 ttgcactttt taatgtgaac aacagcagca ataaggacca ggaaccagaa gaaaaaaaga
721 aaaagaaaaa agaaaagaag agcaagtcag atgataaaaa cgaaaataaa aacgacccag
781 agaagaaaaa gaagaaaaag gacaaagaga agaaaaagaa agaggagaaa agcaaagata
841 agaaagaaga ggagaagaaa gaagttgtgg ttattgatcc ctcgggaaac acatattaca
901 actggctgtt ttgcatcaca ttacctgtta tgtacaactg gacaatggtt attgccagag
961 catgttttga tgaacttcaa tctgattacc tagaatattg gctcattttg gattacgtat
1021 cagacatagt ctatttaatc gatatgtttg tacgaacaag gacaggttac ctagaacaag
1081 gactgctggt aaaggaagaa cttaaactca taaataaata taaatccaac ttgcaattta
1141 aacttgatgt tctgtcactg ataccaactg atttgctgta ttttaagtta gggtggaact
1201 atccagaaat tagattaaac aggttgttac ggttctctcg tatgtttgag ttcttccaga
1261 gaacagaaac aaggacaaac tatccaaaca tcttcaggat ttccaacctt gttatgtata
1321 tcgtcatcat tatccactgg aatgcatgtg tgttctactc tatttctaaa gctattggat
1381 ttggaaatga tacatgggtc taccctgata ttaatgatcc tgaatttggc cgtttggcta
1441 gaaaatacgt atacagcctt tactggtcta cactgacttt gactaccatt ggtgaaacac
1501 cccctcccgt gagggattct gagtatgtct ttgtggtggt tgatttccta attggagtgt
1561 taatttttgc taccatcgtt ggtaacatag gttctatgat ttccaacatg aatgcagcca
1621 gagcagaatt tcaagcaaga attgatgcta tcaagcaata tatgcatttt cgaaatgtaa
1681 gcaaagatat ggaaaagagg gttattaaat ggtttgacta cctgtggacc aacaaaaaaa
1741 cagttgatga gaaagaagtc ttaaagtatc tacctgataa actaagagca gaaattgcca
1801 tcaacgttca cttagacaca ttaaaaaagg tacgcatttt tgctgattgt gaagctggtc
1861 tgttggtgga gttggtcttg aaattgcaac cccaagtcta cagtcctgga gattatattt
1921 gcaagaaagg ggatatcgga cgagagatgt acattatcaa ggaaggcaaa ctcgctgtgg
1981 tggcagatga tggagtcact cagtttgtgg tattgagcga tggcagctac ttcggtgaga
2041 tcagcattct taacattaaa gggagcaaag ctggcaatcg aagaacggcc aatattaaaa
2101 gtattggcta ctcagacctg ttctgtctct caaaagatga cctcatggaa gctctaactg
2161 agtacccaga tgccaaaact atgctggaag agaaagggaa gcagatttta atgaaagatg
2221 gtctactgga tctaaacatt gcaaatgctg gcagtgatcc taaagatctt gaagagaagg
2281 ttactcgaat ggaggggtca gtagacctcc tgcaaaccag gtttgcccga atcttggctg
2341 agtatgagtc catgcagcag aaactgaaac aaagattaac caaggttgag aaatttctga
2401 aaccgcttat tgacacagaa ttttcaagta ttgagggacc tggagcggaa agtgggccca
2461 tcgactctac agattacaag gatgacgacg ataagtagga attccaagct tggcgtaatc
2521 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
2581 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
2641 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
2701 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
2761 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
2821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
2881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
2941 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
3001 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
3061 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
3121 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
3181 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
3241 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
3301 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
3361 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
3421 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
3481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
3541 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
3601 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
3661 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
3721 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
3781 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
3841 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
3901 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
3961 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
4021 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
4081 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
4141 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
4201 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
4261 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
4321 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
4381 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
4441 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
4501 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
4561 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
4621 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
4681 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
4741 tcgtc

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