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pCold-SUMO-10×His-3C-Strep II-FLAG质粒 (pCold系列质粒

货号：KL-ZL-1124-01
别名：pColdSUMO10×His3CStrepIIFLAG 启动子

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL-1124-01	pCold-SUMO-10×His-3C-Strep II-FLAG质粒 (pCold系列质粒	NA		立即咨询	+ -

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pCold-SUMO-10×His-3C-Strep II-FLAG质粒 (pCold系列质粒) 货号：KL-ZL-1124 规格：20ul

别称：	pColdSUMO10×His3CStrepIIFLAG
启动子：	CSPA
复制子：	pBR322
原核抗性：	Amp
克隆菌株：	DH5a
培养条件：	37度
表达宿主：	大肠杆菌
培养条件：	37℃，有氧，LB
诱导方式：	IPTG或乳糖及其类似物

质粒简介：
pCold-SUMO-10×His-3C-Strep II-FLAG质粒是一个可以在低温环境下表达的大肠杆菌载体。

质粒图谱：

序列：

ORIGIN

1 aaggaatggt gtggccgatt aatcataaat atgaaaaata attgttgcat cacccgccaa

61 tgcgtggctt aatgcacatc aaattgtgag cggataacaa tttgatgtgc tagcgcatat

121 ccagtgtagt aaggcaagtc ccttcaagag ttatcgttga tacccctcgt agtgcacatt

181 cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc gaaaggcaca cttaattatt

241 aagaggtaat acaccatgaa tcacaaagtg catatgaatt ggagccaccc gcagttcgaa

301 aaaagcagcg gcagcagcgg cggtcatcac catcatcatc acggcggcag cggcggcagc

361 gggtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct

421 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa

481 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg

541 gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcaggc ccctgaagat

601 ttggacatgg aggataacga tattattgag gctcaccgcg aacagattgg aggcctggag

661 ctcggtaccc tcgagcatca ccatcaccat caccatcacc atcacctgga agttctgttc

721 caggggcccg aattctatgg caggaagaag cggagacagc gacgaagagg atcctggagc

781 cacccgcagt tcgagaaagc ggccgcatat ggcaggaaga agcggagaca gcgacgaaga

841 taagtcgacg attacaagga tgacgacgat aagtaatcta gataggtaat ctctgcttaa

901 aagcacagaa tctaagatcc ctgccattgg gcggggattt ttttatttgt tttcaggaaa

961 taaataatcg atcgcgtaat aaaatctatt attatttttg tgaagaataa atttgggtgc

1021 aatgagaatg cgcaggccct ttcgtctcgc gccgtttcgg tgatgacggt gaaaacctct

1081 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac

1141 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg

1201 catcagagca gattgtactg agagtgcacc ataaaattgt aaacgttaat attttgttaa

1261 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca

1321 aaatccctta taaatcaaaa gaatagcccg agatagggtt gagtgttgtt ccagtttgga

1381 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc

1441 agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc

1501 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc

1561 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg

1621 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac

1681 agggcgcgta ctatggttgc tttgacgtat gcggtgtgaa ataccgcaca gatgcgtaag

1741 gagaaaatac cgcatcaggc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct

1801 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga

1861 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc

1921 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg

1981 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc

2041 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact

2101 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc

2161 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag

2221 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat

2281 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt

2341 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa

2401 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc

2461 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg

2521 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt

2581 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca

2641 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat

2701 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca

2761 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg

2821 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg

2881 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt

2941 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg

3001 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata

3061 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca

3121 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag

3181 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc

3241 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga

3301 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg

3361 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac

3421 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg

3481 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg

3541 ttcctggcct tttgctggcc ttttgctcac atagtcatgc cccgcgccca ccggaaggag

3601 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc

3661 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc

3721 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag

3781 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc

3841 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt

3901 gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac

3961 cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc

4021 catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat

4081 ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat

4141 ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact

4201 taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc

4261 cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac

4321 atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc

4381 atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc

4441 cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag

4501 ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact

4561 ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt

4621 gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac

4681 gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc

4741 gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg

4801 ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct

4861 ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca

4921 ccgccgccgc

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