pAcGP67A质粒 货号：kl-zl-1094 规格：20ul
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启动子： |
Polyhedrin |
|
复制子： |
ColE1 ori |
|
终止子： |
polyhedrin 3 |
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质粒分类： |
昆虫细胞载体；杆状病毒组成型分泌表达质粒 |
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质粒大小： |
9761bp |
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质粒标签： |
N-GP67 signal |
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原核抗性： |
氨苄青霉素Ampicillin |
|
克隆菌株： |
DH5α |
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培养条件： |
37℃，有氧，LB |
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表达宿主： |
昆虫细胞 |
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诱导方式： |
组成性表达，无需诱导 |
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5'测序引物： |
Polyhedrin-F：AAATGATAACCATCTCGC |
|
3'测序引物： |
Polyhedrin-R：GTCCAAGTTTCCCTG |
|
备注： |
含有GP67分泌信号肽 |
质粒简介：
The acidic glycoprotein gp67 (syn.： gp64) is the most abundant envelope surface glycoprotein of the Autographa californica nuclear polyhedrosis virus (AcNPV baculovirus), and is essential for the entry of baculovirus particles into susceptible insect cells. Since large amounts of this protein are secreted and anchored to the virus peplomer, its gene contains one of the most effective baculovirus-encoded signal sequences for protein secretion. Therefore, we have constructed baculovirus transfer vectors that contain the gp67 signal sequence in front of a multiple cloning site. A gene of choice can be inserted in one of these cloning sites and the protein of interest will be expressed as a gp67 signal peptide fusion protein under the control of the strong baculovirus polyhedrin promoter. This strategy allows the forced secretion of otherwise non-secreted recombinant proteins which may be easily purified when serum-free insect culture medium, BD BaculoGold Max-XP Insect Cell Medium is used. The transfer vector(s) should be preferentially used in conjunction with BD BaculoGold DNA .
质粒图谱：
