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pIRESneo3质粒

货号：kl-zl-1073-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-1073-01	pIRESneo3质粒	NA		立即咨询	+ -

产品介绍
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pIRESneo3质粒货号：kl-zl-1073 规格：20ul

启动子：	CMV,T7
复制子：	pUC ori
终止子：	SV40 poly(A) signal
质粒分类：	哺乳细胞，蛋白过表达载体
质粒大小：	5.2kb
原核抗性：	Amp
筛选标记：	Neo
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	哺乳细胞
诱导方式：	无须诱导，瞬时表达
5'测序引物：	CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物：	根据序列设计引物

质粒简介：
pIRESneo3 contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV), which permits the translation of two open reading frames from one messenger RNA (1–3). After selection with G418, nearly all surviving colonies will stably express the gene of interest, thus decreasing the need to screen large numbers of colonies to find functional clones. To select for cells that express high levels of the gene of interest, the selective pressure for antibiotic resistance was increased by shifting the neomycin phosphotransferase gene downstream to a less optimal position for translation as directed by the IRES sequence (1). By decreasing the level of expression of the antibiotic resistance marker, the selective pressure on the entire expression cassette is increased, resulting in the selection of cells that express the entire transcript, including the gene of interest, at high levels.
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