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pDsRed-Monomer-N1质粒

  • 货号:kl-zl-1045-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-1045-01 pDsRed-Monomer-N1质粒 NA

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pDsRed-Monomer-N1质粒 货号:kl-zl-1045 规格:20ul

启动子: CMV
复制子: pUC ori,f1 ori
终止子: SV40 poly(A) signal
质粒分类: 哺乳细胞,荧光蛋白报告载体
质粒大小: 4691bp
原核抗性: Kan
筛选标记: Neo
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'测序引物: 根据序列设计引物
质粒简介:
pDsRed-Monomer-N1 is a mammalian expression vector that encodes DsRed-Monomer (DsRed.M1), a monomeric mutant derived from the tetrameric Discosoma sp. red fuorescent protein DsRed. DsRed-Monomer contains forty-fve amino acid substitutions (listed on page 2). When DsRed-Monomer is expressed in mammalian cell cultures, red fuorescent cells can be detected by either fuorescence microscopy or fow cytometry 12–16 hr after transfection (DsRed-Monomer excitation and emission maxima = 557 nm and 592 nm, respectively). The DsRed-Monomer coding sequence is human codon-optimized for high expression in mammalian cells.
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