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pGL3-Promoter质粒

  • 货号:kl-zl-1030-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-1030-01 pGL3-Promoter质粒 NA

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pGL3-Promoter质粒 货号:kl-zl-1030 规格:20ul

启动子: SV40
复制子: pUC ori,f1 ori,SV40 ori
终止子: SV40 poly(A) signal
质粒分类: 哺乳细胞,信号通路报告载体
质粒大小: 5010bp
原核抗性: Amp
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: RVP3:CTAGCAAAATAGGCTGTCCC
3'测序引物: GLP2:CTTTATGTTTTTGGCGTCTTCC
质粒简介:
The pGL3 Luciferase Reporter Vectors(a–c) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, these Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.
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