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pmirGLO质粒

  • 货号:kl-zl-1017-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-1017-01 pmirGLO质粒 NA

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pmirGLO质粒 货号:kl-zl-1017 规格:20ul

启动子: PGK,SV40
复制子: pUC ori,SV40 ori
终止子: SV40 poly(A) signal
质粒分类: 哺乳细胞,信号通路报告载体
质粒大小: 7350bp
原核抗性: Amp
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: Sv40-polyA-R:GAAATTTGTGATGCTATTGC
3'测序引物: 根据序列设计引物
质粒简介:
The pmirGLO Dual-Luciferase miRNA Target Expression Vector(a–d) is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites 3´ of the firefly luciferase gene (luc2). These target sites can be introduced by cloning putative miRNA binding sites alone, or the 3´ untranslated region (UTR) of a gene of interest, to study the influence of these sites on transcript stability and activity. Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with firefly luciferase (luc2) used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization and selection.
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