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pmirGLO质粒

货号：kl-zl-1017-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-1017-01	pmirGLO质粒	NA		立即咨询	+ -

产品介绍
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pmirGLO质粒货号：kl-zl-1017 规格：20ul

启动子：	PGK,SV40
复制子：	pUC ori，SV40 ori
终止子：	SV40 poly(A) signal
质粒分类：	哺乳细胞，信号通路报告载体
质粒大小：	7350bp
原核抗性：	Amp
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	哺乳细胞
诱导方式：	无须诱导，瞬时表达
5'测序引物：	Sv40-polyA-R：GAAATTTGTGATGCTATTGC
3'测序引物：	根据序列设计引物

质粒简介：
The pmirGLO Dual-Luciferase miRNA Target Expression Vector(a–d) is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites 3´ of the firefly luciferase gene (luc2). These target sites can be introduced by cloning putative miRNA binding sites alone, or the 3´ untranslated region (UTR) of a gene of interest, to study the influence of these sites on transcript stability and activity. Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with firefly luciferase (luc2) used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization and selection.
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