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pAAV-RC pAAV-RC2腺相关病毒包装质粒

货号：kl-zl-0985-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0985-01	pAAV-RC pAAV-RC2腺相关病毒包装质粒	NA		立即咨询	+ -

产品介绍
相关产品

pAAV-RC质粒货号：kl-zl-0985 规格：3ug

别名：	pAAV-RC；pAAV-RC2
复制子：	pUC ori
质粒分类：	腺相关病毒包装质粒
质粒大小：	7327bp
原核抗性：	氨苄青霉素Amp
克隆菌株：	大肠杆菌Stbl3
培养条件：	37℃，有，LB
表达宿主：	哺乳细胞
5'测序引物：	M13F：TGTAAAACGACGGCCAGT
3'测序引物：	M13R：CAGGAAACAGCTATGACC

质粒简介：
The pAAV-RC plasmid contains the AAV-2 rep and cap genes, encoding replication proteins and viral capsid structural proteins, respectively. Establishing the proper expression levels of rep and cap gene products is an important step in achieving high-titer virus production. pAAV-RC directs Rep and Cap expression from two different promoters, achieving optimal expression levels for each set of gene products.
质粒图谱：

序列：

ORIGIN

1 gcgcgccgat atcgttaacg ccccgcgccg gccgctctag aactagtgga tcccccggaa

61 gatcagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttctgatctg

121 cgcagccgcc atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga

181 gcatctgccc ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt

241 gccgccagat tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga

301 gaagctgcag cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggccct

361 tttctttgtg caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac

421 caccggggtg aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat

481 tcagagaatt taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac

541 cagaaatggc gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt

601 gctccccaaa acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag

661 cgcctgtttg aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc

721 gcagacgcag gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag

781 atcaaaaact tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac

841 ctcggagaag cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc

901 caactcgcgg tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac

961 taaaaccgcc cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg

1021 gatttataaa attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct

1081 gggatgggcc acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac

1141 taccgggaag accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt

1201 aaactggacc aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg

1261 ggaggagggg aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag

1321 caaggtgcgc gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat

1381 cgtcacctcc aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca

1441 ccagcagccg ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga

1501 ctttgggaag gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt

1561 ggttgaggtg gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc

1621 cagtgacgca gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac

1681 gtcagacgcg gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca

1741 cgtgggcatg aatctgatgc tgtttccctg cagacaatgc gagagaatga atcagaattc

1801 aaatatctgc ttcactcacg gacagaaaga ctgtttagag tgctttcccg tgtcagaatc

1861 tcaacccgtt tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcatatcat

1921 gggaaaggtg ccagacgctt gcactgcctg cgatctggtc aatgtggatt tggatgactg

1981 catctttgaa caataaatga tttaaatcag gtatggctgc cgatggttat cttccagatt

2041 ggctcgagga cactctctct gaaggaataa gacagtggtg gaagctcaaa cctggcccac

2101 caccaccaaa gcccgcagag cggcataagg acgacagcag gggtcttgtg cttcctgggt

2161 acaagtacct cggacccttc aacggactcg acaagggaga gccggtcaac gaggcagacg

2221 ccgcggccct cgagcacgac aaagcctacg accggcagct cgacagcgga gacaacccgt

2281 acctcaagta caaccacgcc gacgcggagt ttcaggagcg ccttaaagaa gatacgtctt

2341 ttgggggcaa cctcggacga gcagtcttcc aggcgaaaaa gagggttctt gaacctctgg

2401 gcctggttga ggaacctgtt aagacggctc cgggaaaaaa gaggccggta gagcactctc

2461 ctgtggagcc agactcctcc tcgggaaccg gaaaggcggg ccagcagcct gcaagaaaaa

2521 gattgaattt tggtcagact ggagacgcag actcagtacc tgacccccag cctctcggac

2581 agccaccagc agccccctct ggtctgggaa ctaatacgat ggctacaggc agtggcgcac

2641 caatggcaga caataacgag ggcgccgacg gagtgggtaa ttcctcggga aattggcatt

2701 gcgattccac atggatgggc gacagagtca tcaccaccag cacccgaacc tgggccctgc

2761 ccacctacaa caaccacctc tacaaacaaa tttccagcca atcaggagcc tcgaacgaca

2821 atcactactt tggctacagc accccttggg ggtattttga cttcaacaga ttccactgcc

2881 acttttcacc acgtgactgg caaagactca tcaacaacaa ctggggattc cgacccaaga

2941 gactcaactt caagctcttt aacattcaag tcaaagaggt cacgcagaat gacggtacga

3001 cgacgattgc caataacctt accagcacgg ttcaggtgtt tactgactcg gagtaccagc

3061 tcccgtacgt cctcggctcg gcgcatcaag gatgcctccc gccgttccca gcagacgtct

3121 tcatggtgcc acagtatgga tacctcaccc tgaacaacgg gagtcaggca gtaggacgct

3181 cttcatttta ctgcctggag tactttcctt ctcagatgct gcgtaccgga aacaacttta

3241 ccttcagcta cacttttgag gacgttcctt tccacagcag ctacgctcac agccagagtc

3301 tggaccgtct catgaatcct ctcatcgacc agtacctgta ttacttgagc agaacaaaca

3361 ctccaagtgg aaccaccacg cagtcaaggc ttcagttttc tcaggccgga gcgagtgaca

3421 ttcgggacca gtctaggaac tggcttcctg gaccctgtta ccgccagcag cgagtatcaa

3481 agacatctgc ggataacaac aacagtgaat actcgtggac tggagctacc aagtaccacc

3541 tcaatggcag agactctctg gtgaatccgg gcccggccat ggcaagccac aaggacgatg

3601 aagaaaagtt ttttcctcag agcggggttc tcatctttgg gaagcaaggc tcagagaaaa

3661 caaatgtgga cattgaaaag gtcatgatta cagacgaaga ggaaatcagg acaaccaatc

3721 ccgtggctac ggagcagtat ggttctgtat ctaccaacct ccagagaggc aacagacaag

3781 cagctaccgc agatgtcaac acacaaggcg ttcttccagg catggtctgg caggacagag

3841 atgtgtacct tcaggggccc atctgggcaa agattccaca cacggacgga cattttcacc

3901 cctctcccct catgggtgga ttcggactta aacaccctcc tccacagatt ctcatcaaga

3961 acaccccggt acctgcgaat ccttcgacca ccttcagtgc ggcaaagttt gcttccttca

4021 tcacacagta ctccacggga caggtcagcg tggagatcga gtgggagctg cagaaggaaa

4081 acagcaaacg ctggaatccc gaaattcagt acacttccaa ctacaacaag tctgttaatg

4141 tggactttac tgtggacact aatggcgtgt attcagagcc tcgccccatt ggcaccagat

4201 acctgactcg taatctgtaa ttgcttgtta atcaataaac cgtttaattc gtttcagttg

4261 aactttggtc tctgcgtatt tctttcttat ctagtttcca tggctacgta gataagtagc

4321 atggcgggtt aatcattaac tacagcccgg gcgtttaaac agcgggcgga ggggtggagt

4381 cgtgacgtga attacgtcat agggttaggg aggtcctgta ttagaggtca cgtgagtgtt

4441 ttgcgacatt ttgcgacacc atgtggtctc gctggggggg ggggcccgag tgagcacgca

4501 gggtctccat tttgaagcgg gaggtttgaa cgagcgctgg cgcgctcact ggccgtcgtt

4561 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat

4621 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag

4681 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt

4741 taaatttttg ttaaatcagc tcattttttt aaccaatagg ccgaaatcgg caaaatccct

4801 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt

4861 ccactattaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg

4921 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac

4981 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg

5041 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag

5101 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt

5161 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac

5221 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa

5281 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat

5341 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc

5401 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga

5461 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg

5521 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc

5581 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag

5641 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc

5701 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg

5761 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg

5821 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac

5881 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac

5941 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg

6001 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg

6061 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg

6121 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac

6181 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg

6241 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg

6301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc

6361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc

6421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt

6481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc

6541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact

6601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac

6661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag

6721 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg

6781 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg

6841 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga

6901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt

6961 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct

7021 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg

7081 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt

7141 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta

7201 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta

7261 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt

7321 acgccaa

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