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pLVX-TRE3G-Zsgreen1质粒

  • 货号:kl-zl-0953-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0953-01 pLVX-TRE3G-Zsgreen1质粒 NA

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pLVX-TRE3G-Zsgreen1质粒 货号:kl-zl-0953 规格:20ul

启动子: TRE3GV,PGK
复制子: pUC ori
终止子: SV40 poly(A) signal
质粒分类: 病毒系列,慢病毒克隆载体
质粒大小: 9100bp
原核抗性: Amp
筛选标记: Puro
克隆菌株: Stbl3
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 四环素诱导
5'测序引物: M13R:CAGGAAACAGCTATGACC
3'测序引物: 根据序列设计引物
质粒简介:
The inducible promoter PTRE3G provides for very low basal expression and high maximal expression after induction. It consists of 7 repeats of a 19 bp tet operator sequence located upstream of a minimal CMV promoter. PTRE3GV is a version of PTRE3G that was modified at Clontech for higher performance in lentiviruses and retroviruses. In the presence of Dox, Tet-On 3G binds specifically to PTRE3GV and activates transcription of the downstream GOI. PTRE3GV lacks binding sites for endogenous mammalian transcription factors, so it is virtually silent in the absence of induction. pLVX-TRE3G-ZsGreen1 is an HIV-1-based, inducible lentiviral expression vector that allows the simultaneous expression of ZsGreen1 and your protein of interest in virtually any mammalian cell type, including primary cells.
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