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pPinkα-HC质粒

货号：kl-zl-0893-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0893-01	pPinkα-HC质粒	NA		立即咨询	+ -

产品介绍
相关产品

pPinkα-HC质粒货号：kl-zl-0893 规格：20ul

启动子：	PpADE2 HC，AOX1
复制子：	pUC ori
终止子：	CYC1
质粒分类：	酵母系列，毕赤酵母表达载体
质粒大小：	7898bp
原核抗性：	Amp
筛选标记：	ADE2
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	酵母细胞
5'测序引物：	AOX5：GACTGGTTCCAATTGACAAGC
3'测序引物：	AOX3：GGCAAATGGCATTCTGACAT

质粒简介：
The PichiaPink™ Secreted Vector Kit contains the pPINKα-HC vector for use with the PichiaPink™ Yeast Expression System. The pPINKα-HC vector is built around the ADE2 gene for complementing the complementing the ade2 deletion in the PichiaPink™ strains. The pPINKα-HC vector has the α-mating factor secretion signal sequence so that all proteins are secreted. You can order the PichiaPink™ Secreted Vector Kit to refill your PichiaPink™ Yeast Expression System Kit.
质粒图谱：

promoter complement(7298..7402)

/gene="bla"

/note="AmpR promoter"

ORIGIN

1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag

61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt

121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc

181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta

241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta

301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg

361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct

421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg

481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt

541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct

601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct

661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact

721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat

781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt

841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga

901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt

961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga

1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga

1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa

1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga aaaggcctca

1201 ggtaccggcc ggccatttaa atacaggccc cttttccttt gtcgatatca tgtaattagt

1261 tatgtcacgc ttacattcac gccctcctcc cacatccgct ctaaccgaaa aggaaggagt

1321 tagacaacct gaagtctagg tccctattta ttttttttaa tagttatgtt agtattaaga

1381 acgttattta tatttcaaat ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac

1441 attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcaag

1501 ctggatccgc ggccgccttc caaactctca tggattctca ggtaataggt attctaggag

1561 gaggccagct aggccgaatg attgttgagg ccgctagcag gctcaatatc aagaccgtga

1621 ttcttgatga tggtttttca cctgctaagc acattaatgc tgcgcaagac cacatcgacg

1681 gatcattcaa agatgaggag gctatcgcca agttagctgc caaatgtgat gttctcactg

1741 tagagattga gcatgtcaac acagatgctc taaagagagt tcaagacaga actggaatca

1801 agatatatcc tttaccagag acaatcgaac taatcaagga taagtacttg caaaaggaac

1861 atttgatcaa gcacaacatt tcggtgacaa agtctcaggg tatagaatct aatgaaaagg

1921 cgctgctttt gtttggagaa gagaatggat ttccatatct gttgaagtcc cggactatgg

1981 cttatgatgg aagaggcaat tttgtagtgg agtctaaaga ggacatcagt aaggcattag

2041 agttcttgaa agatcgtcca ttgtatgccg agaagtttgc tccttttgtt aaagaattag

2101 cggtaatggt tgtgagatca ctggaaggcg aagtattctc ctacccaacc gtagaaactg

2161 tgcacaagga caatatctgt catattgtgt atgctccggc cagagttaat gacaccatcc

2221 aaaagaaagc tcaaatatta gctgaaaaca ctgtgaagac tttcccaggc gctggaatct

2281 tcggagttga gatgttccta ttgtctgatg gagaacttct tgtaaatgag attgctccaa

2341 ggccccacaa ttctggtcac tatacaatcg atgcatgtgt aacatctcag ttcgaagcac

2401 atgtaagagc cataactggt ctgccaatgc cactagattt caccaaacta tctacttcca

2461 acaccaacgc tattatgctc aatgttttgg gtgctgaaaa atctcacggg gaattagagt

2521 tttgtagaag agccttagaa acacccggtg cttctgtata tctgtacgga aagaccaccc

2581 gattggctcg taagatgggt catatcaaca taataggatc ttccatgttg gaagcagaac

2641 aaaagttaga gtacattcta gaagaatcaa cccacttacc atccagtact gtatcagctg

2701 acactaaacc gttggttgga gttatcatgg gttcagactc tgatctacct gtgatttcga

2761 aaggttgcga tattttaaaa cagtttggtg ttccattcga agttactatt gtctctgctc

2821 atagaacacc acagagaatg accagatatg cctttgaagc cgctagtaga ggtatcaagg

2881 ctatcattgc aggtgctggt ggtgctgctc atcttccagg aatggttgct gccatgactc

2941 cgttgccagt cattggtgtt cctgtcaagg gctctacgtt ggatggtgta gactcgctac

3001 actcgattgt ccaaatgcct agaggtgttc ctgtggctac ggttgctatc aacaacgcca

3061 ccaatgccgc tctgttggcc atcaggattt taggtacaat tgaccacaaa tggcaaaagg

3121 aaatgtccaa gtatatgaat gcaatggaga ccgaagtgtt ggggaaggca tccaacttgg

3181 aatctgaagg gtatgaatcc tatttgaaga atcgtctttg aatttagtat tgttttttaa

3241 tagatgtata tataatagta cacgtaactt atctattcca ttcataattt tattttaaag

3301 gttcggtaga aatttgtcct ccaaaaagtt ggttagagcc tggcagtttt gataggcatt

3361 attatagatt gggtaatatt taccctgcac ctggaggaac tttgcaaaga gcctcatgtg

3421 cggcgcgcca ggccataatg gccaaacggt ttctcaatta ctatatacta ctaaccattt

3481 acctgtagcg tatttctttt ccctcttcgc gaaagctcaa gggcatcttc ttgactcatg

3541 aaaaatatct ggatttcttc tgacagatca tcacccttga gcccaactct ctagcctatg

3601 agtgtaagtg atagtcatct tgcaacagat tattttggaa cgcaactaac aaagcagata

3661 cacccttcag cagaatcctt tctggatatt gtgaagaatg atcgccaaag tcacagtcct

3721 gagacagttc ctaatcttta ccccatttac aagttcatcc aatcagactt cttaacgcct

3781 catctggctt atatcaagct taccaacagt tcagaaactc ccagtccaag tttcttgctt

3841 gaaagtgcga agaatggtga caccgttgac aggtacacct ttatgggaca ttcccccaga

3901 aaaataatca agactgggcc tttagagggt gctgaagttg accccttggt gcttctggaa

3961 aaagaactga agggcaccag acaagcgcaa cttcctggta ttcctcgtct aagtggtggt

4021 gccataggat acatctcgta cgattgtatt aagtactttg aaccaaaaac tgaaagaaaa

4081 ctgaaagatg ttttgcaact tccggaagca gctttgatgt tgttcgacac gatcgtggct

4141 tttgacaatg tttatcaaag attccaggta attggaaacg tttctctatc cgttgatgac

4201 tcggacgaag ctattcttga gaaatattat aagacaagag aagaagtgga aaagatcagt

4261 aaagtggtat ttgacaataa aactgttccc tactatgaac agaaagatat tattcaaggc

4321 caaacgttca cctctaatat tggtcaggaa gggtatgaaa accatgttcg caagctgaaa

4381 gaacatattc tgaaaggaga catcttccaa gctgttccct ctcaaagggt agccaggccg

4441 acctcattgc accctttcaa catctatcgt catttgagaa ctgtcaatcc ttctccatac

4501 atgttctata ttgactatct agacttccaa gttgttggtg cttcacctga attactagtt

4561 aaatccgaca acaacaacaa aatcatcaca catcctattg ctggaactct tcccagaggt

4621 aaaactatcg aagaggacga caattatgct aagcaattga agtcgtcttt gaaagacagg

4681 gccgagcacg tcatgctggt agatttggcc agaaatgata ttaaccgtgt gtgtgagccc

4741 accagtacca cggttgatcg tttattgact gtggagagat tttctcatgt gatgcatctt

4801 gtgtcagaag tcagtggaac attgagacca aacaagactc gcttcgatgc tttcagatcc

4861 attttcccag caggaaccgt ctccggtgct ccgaaggtaa gagcaatgca actcatagga

4921 gaattggaag gagaaaagag aggtgtttat gcgggggccg taggacactg gtcgtacgat

4981 ggaaaatcga tggacacatg tattgcctta agaacaatgg tcgtcaagga cggtgtcgct

5041 taccttcaag ccggaggtgg aattgtctac gattctgacc cctatgacga gtacatcgaa

5101 accatgaaca aaatgagatc caacaataac accatcttgg aggctgagaa aatctggacc

5161 gataggttgg ccagagacga gaatcaaagt gaatccgaag aaaacgatca atgaacggag

5221 gacgtaagta ggaatttatg gtttggccat aatggcctag cttggcgtaa tcatggtcat

5281 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa

5341 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc

5401 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc

5461 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact

5521 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac

5581 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa

5641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg

5701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa

5761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc

5821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac

5881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac

5941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg

6001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt

6061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga

6121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct

6181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga

6241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg

6301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct

6361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt

6421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc

6481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg

6541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag

6601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt

6661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag

6721 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt

6781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca

6841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg

6901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat

6961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta

7021 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca

7081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct

7141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat

7201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa

7261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt

7321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa

7381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa

7441 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg

7501 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag

7561 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg

7621 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc

7681 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt

7741 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac

7801 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt

7861 cccagtcacg acgttgtaaa acgacggcca gtgaattg

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