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pGBKT7-lam质粒

  • 货号:kl-zl-0882-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0882-01 pGBKT7-lam质粒 NA

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pGBKT7-lam质粒 货号:kl-zl-0882 规格:20ul

启动子: T7,ADH1
复制子: 2μ ori,ori,FI ori
质粒分类: 酵母系列,酵母双杂交载体
质粒大小: 7.9kb
原核抗性: Kan
筛选标记: TRP1
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 酵母细胞
5'测序引物: T7:TAATACGACTCACTATAGGG
3'测序引物: 根据序列设计引物
质粒简介:
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.
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