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pHIS2质粒

货号：kl-zl-0876-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0876-01	pHIS2质粒	NA		立即咨询	+ -

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pHIS2质粒货号：kl-zl-0876 规格：20ul

启动子：	TRP1 ,T7,T3
复制子：	pUC ori，FI ori
质粒分类：	酵母系列，酵母单杂交载体
质粒大小：	7207bp
原核抗性：	Kan
筛选标记：	TRP1
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	酵母细胞
5'测序引物：	M13F：TGTAAAACGACGGCCAGT
3'测序引物：	根据序列设计引物

质粒简介：
pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene,located downstream of a multiple cloning site (MCS) and the minimal promoter of the HIS3 locus(PminHIS3). Cis-acting DNA sequences, or DNA target elements, can be inserted into the MCS andused as baits to screen GAL4 AD/cDNA fusion libraries for proteins that interact with the targetsequence. A protein-DNA (or one-hybrid) interaction can be detected by performing the assay in ayeast strain such as Y187 that is auxotrophic for histidine. Positive one-hybrid interactions driveexpression of the HIS3 reporter gene, which enables the host cell to grow on histidine-deficientmedia.
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