您当前的位置是：

pHIS2质粒

货号：kl-zl-0876-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0876-01	pHIS2质粒	NA		立即咨询	+ -

产品介绍
相关产品

pHIS2质粒货号：kl-zl-0876 规格：20ul

启动子：	TRP1 ,T7,T3
复制子：	pUC ori，FI ori
质粒分类：	酵母系列，酵母单杂交载体
质粒大小：	7207bp
原核抗性：	Kan
筛选标记：	TRP1
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	酵母细胞
5'测序引物：	M13F：TGTAAAACGACGGCCAGT
3'测序引物：	根据序列设计引物

质粒简介：
pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene,located downstream of a multiple cloning site (MCS) and the minimal promoter of the HIS3 locus(PminHIS3). Cis-acting DNA sequences, or DNA target elements, can be inserted into the MCS andused as baits to screen GAL4 AD/cDNA fusion libraries for proteins that interact with the targetsequence. A protein-DNA (or one-hybrid) interaction can be detected by performing the assay in ayeast strain such as Y187 that is auxotrophic for histidine. Positive one-hybrid interactions driveexpression of the HIS3 reporter gene, which enables the host cell to grow on histidine-deficientmedia.
质粒图谱：

货号	产品名称	价格	数量
kl-zl-0640-01	pFLAG-CMV2质粒	立即咨询	+ -
kl-zl-0441-01	pET28a-Ngal质粒	立即咨询	+ -
kl-zl-0914-01	pBBR1MCS-1质粒	立即咨询	+ -
kl-zl-0865-01	pHH43质粒	立即咨询	+ -
kl-zl-0820-01	pET28a-OFP质粒	立即咨询	+ -
kl-zl-0934-01	pBE2-R质粒	立即咨询	+ -
kl-zl-0519-01	pDONR223-IDH1质粒	立即咨询	+ -
kl-zl-0257-01	pCMV-Myc-C质粒	立即咨询	+ -
KL-ZL-1203-01	GSK3B(NM_001354596.1)人源基因质粒	立即咨询	+ -
kl-zl-740-01	pQE-30Xa质粒	立即咨询	+ -
kl-zl-0739-01	pQE-30质粒	立即咨询	+ -
kl-zl-0785-01	pYM30-ECFP质粒	立即咨询	+ -