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pRS426质粒

  • 货号:kl-zl-0839-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0839-01 pRS426质粒 NA

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pRS426质粒 货号:kl-zl-0839 规格:20ul

启动子: URA3,T7,T3
复制子: 2μ ori,ori,FI ori
质粒分类: 酵母系列,酿酒酵母表达载体
质粒大小: 5726bp
原核抗性: Amp
筛选标记: URA3
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 酵母细胞
诱导方式: 半乳糖诱导
5'测序引物: M13R:CAGGAAACAGCTATGACC
3'测序引物: 根据序列设计引物
质粒简介:
The order of the major features in this plasmid is:ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori (2 micron) - URA3. Contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.4 + or - 1.4% of progeny per doubling. YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; PstI--4.0, 1.7; PvuII--5.2, 0.54. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416322 Hosts: E.coli DH5alpha, Saccharomyces cerevisiae, E.coli.
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