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pBI221-EGFP质粒

货号：kl-zl-0833-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0833-01	pBI221-EGFP质粒	NA		立即咨询	+ -

产品介绍
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pBI221-EGFP质粒货号：kl-zl-0833 规格：20ul

启动子：	CaMV 35S ，Lac
复制子：	ori
终止子：	NOS
质粒分类：	植物系列，蛋白过表达载体
质粒大小：	4530bp
原核抗性：	Amp
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
5'测序引物：	35S：GACGCACAATCCCACTATCC
3'测序引物：	根据序列设计引物

质粒简介：
Most studies related to determining the expression profile of genes and specific promoters used histochemical localization of the reporter gene, gusA. While the histochemical method for visualizing gusA expression suffers from several limitations in the determination of gene expression and location, especially in the tissues with high background acitivty. To solve this problem, a transient expession vector pBI221-RFP/GFP, was constructed using GFP and RFP as double fluorescent marker genes. This vector used CaMV 35S promoter to drive GFP and determine the transforming efficiency. It analyzed expression profile of the target gene and promoter through the RFP activities of the tranformed tissues. Through the specific promoter AGPL1 from watermelon and E8 promoter from tomato, it is resistible to use this vector to study the expression patterns of promoters. Results indicated that the pBI221-RFP/GFP is a very efficient transient expression vector that can be verify the functions of the genes and promoters.
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