pCAMBIA0380质粒 货号：kl-zl-0831 规格：20ul
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复制子： |
pVS1 oriV，ori |
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终止子： |
NOS |
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质粒分类： |
植物系列，蛋白过表达载体 |
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质粒大小： |
6813bp |
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质粒标签： |
6xHis |
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原核抗性： |
Kan |
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克隆菌株： |
DH5α |
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培养条件： |
37℃，有氧 LB |
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表达宿主： |
植物细胞 |
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5'测序引物： |
根据序列设计引物 |
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3'测序引物： |
根据序列设计引物 |
质粒简介：
Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal. NOTE that this 35S promoter can have an enhancer effect on the expression of other genes in the same cassette, so gene expression results using pCambia derivatives in which portions of this promoter are still present should be interpreted with caution. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins. The sequence for this tag occurs between the first NheI site (there is a second NheI site in the pVS1-rep that we didn't eliminate) and the unique PmlI site. Genes of interest may be inserted in place of the reporter gene. Insertion without a stop codon and in frame at the (first) NheI site will append a hexa-Histidine tag to your protein of interest. Insertion without a stop codon and in frame at the PmlI site will append a stop codon. Insertion at the BstEII site will add neither a tag nor a stop codon (so you may want to ensure that a sequence inserted here contains a stop codon).
质粒图谱：
