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pTet-On质粒

  • 货号:kl-zl-0771-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0771-01 pTet-On质粒 NA

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pTet-On质粒 货号:kl-zl-0771 规格:20ul

启动子: CMV
复制子: pUC ori
终止子: SV40 poly(A) signal,beta-globin polyA signal
质粒分类: 哺乳细胞,四环素调控系统载体
质粒大小: 7400bp
原核抗性: Amp
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 四环素诱导
5'测序引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'测序引物: 根据序列设计引物
质粒图谱:
pTet-On® expresses the reverse tet-responsive transcriptional activator (rtTA) from the strong immediate early promoter of cytomegalovirus (PCMV). tTA is a fusion of amino acids 1–207 of the tet repressor (TetR) and the negatively charged C-terminal activation domain (130 amino acids) of the VP16 protein of herpes simplex virus. pTet-On is similar to pTet-Off except for four amino acid changes that convert the TetR to a rTetR, and consequently, tTA to rtTA. (pTet-Off also contains several silent mutations.) pTet-On was originally described as pUHD17-1neo by Gossen et al. (1995). pTet-On can be distinguished from pTet-Off by digestion with Hind III.
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