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pJQ200SK质粒

货号：kl-zl-0693-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0693-01	pJQ200SK质粒	NA		立即咨询	+ -

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pJQ200SK质粒货号：kl-zl-0693 规格：20ul

质粒类型：	基因置换载体（自杀型）
克隆方法：	多克隆位点，限制性内切酶
载体抗性：	Gentamicin (庆大霉素)

质粒简介：
Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration. Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts. When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector. A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g., as a BamHI cassette). The order of the major features of the plasmid is： gtmR - SstI/MCS/KpnI - P15A - traJ - oriT - sacB. [1] Deposited by： M.F.Hynes Grwoth media： LB plus gentamicin (ATCC medium number 1885) 37C Hosts： E.coli.
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