| 货号 | 产品名称 | 规格 | 库存 | 价格 | 数量 | 购买 |
|---|---|---|---|---|---|---|
| kl-zl-0661-01 | pMal-p5X质粒 | NA |
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pMal-p5X质粒 货号：kl-zl-0661 规格：20ul
| 启动子： | Tac |
|---|---|
| 复制子： | ColE1 ori |
| 终止子： | rrnB T1 terminator |
| 质粒分类： | 大肠杆菌载体；pMal系列表达质粒 |
| 质粒大小： | 5752bp |
| 质粒标签： | N-MBP |
| 原核抗性： | 氨苄青霉素Amp |
| 克隆菌株： | DH5α |
| 培养条件： | 37℃，有氧，LB |
| 表达宿主： | BL21(DE3) |
| 诱导方式： | IPTG或乳糖及其类似物 |
| 5'测序引物： | 根据序列设计 |
| 3'测序引物： | 根据序列设计 |
| 备注： | 融合表达麦芽糖结合蛋白MBP，蛋白定位于细胞周质 |
The vector pMAL-p5X is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Factor Xa (NEB #P8010).
MBP fusions made with this vector include an N-terminal signal sequence, so the fusion protein is directed to the periplasm. The MBP has been engineered for tighter binding to amylose resin.
A gene or open reading frame is inserted into a restriction site of the vector polylinker, in the same translational reading frame as themalEgene (encoding maltose-binding protein). The fusion protein thus produced can be purified by amylose affinity chromatography. The sequence coding for the four amino acids Ile-Glu-Gly-Arg is present just upstream of the XmnI site. This allows the protein of interest to be cleaved from maltose-binding protein with the specific protease Factor Xa. Fragments inserted in the XmnI site (cleaves GAAGG↓ATTTC) will produce a fusion protein that, after Factor Xa cleavage, contains no vector-derived residues on the protein of interest.
质粒图谱：
