| 货号 | 产品名称 | 规格 | 库存 | 价格 | 数量 | 购买 |
|---|---|---|---|---|---|---|
| kl-zl-0282-01 | pGluc-Basic质粒 | NA |
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pGluc-Basic质粒 货号：kl-zl-0282
| 质粒类型： | 启动子增强子报告载体 |
|---|---|
| 启动子： | 无 |
| 克隆方法： | 多克隆位点，限制性内切酶 |
| 载体大小： | 4920 bp |
| 5' 测序引物及序列： | 5′ d(GGGGTTCCGCGCACATTTCCCCG) 3′ |
| 载体抗性： | Ampicillin (氨苄青霉素) |
pGLuc-Basic is a plasmid cloning vector capable both of replication in E. coli and stable transfection of mammalian cells. It is designed for the cloning of promoter sequences and measurement of their transcription activity using the Gaussia Luciferase Assay Kit (NEB #E3300).
In E. coli, it replicates using the pMB1 origin of replication from pBR322 (although the rop gene is missing) and carries the bla (ApR) marker for selection with ampicillin. It also carries the nptII (NmR) marker under control of an SV40 promoter; thus, following transfection into mammalian cells, it can be used to form stable cell lines by selection with geneticin (G418).
The multiple cloning site (MCS) is positioned immediately upstream of a promoterless reporter gene, GLuc (the humanized coding sequence for the secreted Gaussia princeps luciferase) (1), which is followed by a synthetic polyadenylation (polyA) sequence (not shown). Thus, in mammalian cells the transcriptional activity of promoter sequences cloned into the MCS can be assessed by measuring GLuc activity in the culture medium.
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