| 货号 | 产品名称 | 规格 | 库存 | 价格 | 数量 | 购买 |
|---|---|---|---|---|---|---|
| kl-zl-0265-01 | pCEP4质粒 | NA |
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pCEP4质粒 货号： kl-zl-0265
| 质粒类型： | 哺乳动物表达载体 |
|---|---|
| 启动子： | CMV |
| 表达水平： | 高 |
| 克隆方法： | 多克隆位点，限制性内切酶 |
| 载体大小： | 10186 bp |
| 5' 测序引物： | CMV-F： 5'-CGCAAATGGGCGGTAGGCGTG-3' |
| 3' 测序引物： | EBV-R：5'-GTGGTTTGTCCAAACTCATC-3' |
| 载体抗性： | Ampicillin (氨苄青霉素) |
| 筛选标记： | Hygromycin (潮霉素) |
pCEP4 is an episomal mammalian expression vector that uses the cytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells. pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.
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