| 货号 | 产品名称 | 规格 | 库存 | 价格 | 数量 | 购买 |
|---|---|---|---|---|---|---|
| kl-zl-0256-01 | pCMV-HA-C质粒 | NA |
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pCMV-HA-C质粒 货号：kl-zl-0256
| 质粒类型： | 哺乳动物表达载体 |
|---|---|
| 高拷贝/低拷贝： | 高拷贝 |
| 启动子： | CMV |
| 克隆方法： | 多克隆位点，限制性内切酶 |
| 载体大小： | 3769 bp |
| 5' 测序引物及序列： | pCMV-F： 5'd[GATCCGGTACTAGAGGAACTGAAAAAC]3' |
| 载体标签： | N-HA |
| 载体抗性： | Ampicillin (氨苄青霉素) |
To create a fusion of a gene of interest and the HA tag, insert the gene into the MCS in frame with the HA coding sequence. The resulting HA-tagged proteins can be identified with the HA-Tag Polyclonal Antibody (IgG), provided with this vector, or another antibody raised against the HA tag. The epitope tag is also useful for facilitating purification of the protein, identifying associated proteins, characterizing new proteins by immunoprecipitation, and determining subcellular localization. The MCS in this vector is compatible with the MCSs in Clontech's Matchmaker™ Two-Hybrid System Vectors. Compatibility with System 3 Vectors is noted in the MCS diagram. Consult the Vector Information Packet provided with any Matchmaker vector for complete information.
After obtaining putative positive clones in your Matchmaker two-hybrid screen, use the pCMV-Myc and pCMV-HA Vectors to verify the interactions identified in yeast directly in mammalian cells. To accomplish this, subclone the selected inserts into the pCMV-Myc Vector and the "bait" insert into the pCMV-HA Vector. Alternatively, clone the "bait" insert into pCMV-Myc and the library inserts into pCMV-HA. To confirm predicted interactions in vivo via coimmunoprecipitation, cotransfect pCMV-Myc with the pCMV-HA Vector into mammalian cells and immunoprecipitate using the c-Myc Monoclonal or HA-Tag Polyclonal Antibody provided with the vectors.
质粒图谱：
